花生幼叶为外植体的植株再生系统的建立  

Establishing a High Frequency Plant Regeneration System from Young Leaflets of Peanut (Arachis hypogaea L.)

作　　者：林荣双[1] 梁丽琨[1] 肖显华[1] 王顺珍[1] 

机构地区：[1]山东省烟台大学生物化学系,烟台264005

出　　处：《植物学通报》2003年第3期307-312,共6页Chinese Bulletin of Botany

基　　金：山东省自然科学基金资助项目(Y97D160 75)

摘　　要：本文报道利用花生成熟胚幼叶为外植体获得高频植株再生的方法 ,为花生转基因提供有效的受体系统。通过诱导培养基TDZ、BA、NAA的浓度以及种子萌发时间、继代培养基种类五个因素不同水平的正交试验 ,筛选出了分化高频发生的最佳组合为 :MS培养基中应含有TDZ 1 .0 μmol/L、BA 0 .4μmol/L、NAA 5 .0 μmol/L ,种子萌发 4d,继代培养基为MS0 。本研究表明 ,五因素中诱导培养基TDZ浓度为诱导花生幼叶分化的主要影响因素 ,其次为继代培养基、种子萌发时间 ,而诱导培养基中BA和NAA的浓度作用较小。试管苗生根后移栽田间 。This paper describes a protocol for high frequency regeneration from young leaflets of peanut ( Arachis hypogaea L.) mature embryos, which provides a effective regeneration system for gene transformation. Through orthogonal experiments with different levels of five factors which were TDZ concentration, BA concentration and NAA concentration in the MS medium, germinating days of seeds and subculture medium, we have obtained optimum conditions for regeneration of plantlets with high frequency. The conditions were TDZ 1.0 μmol/L, BA 0.4 μmol/L, NAA 5.0 μmol/L in the MS medium, germination for 4 days and subculture medium MS 0. Among the five factors, TDZ was believed to play key role in inducing differentiation of peanut young leaflets while the subculture medium and germination days came next. The BA and NAA concentration were the least important factors in inducing differentiation. The multiple buds developed further into plants and rooted on medium containing 1/2 MS major elements, 2% sucrose and 5.0 μmol/L IBA. After being transferred onto soil the plants flowered and bore fruits.

关 键 词：花生 幼叶 外植体 植株再生 

分 类 号：S565.2[农业科学—作物学]