转Bt基因抗虫棉对中华草蛉发育及繁殖的影响研究  

Impacts of transgenic Bt cotton on the development and fecundity of Chrysopa sinica Tjeder.

作　　者：董亮[1] 万方浩[1] 张桂芬[1] 刘小京[2] 李强[3] 

机构地区：[1]中国农业科学院生物防治研究所农业部生物防治资源研究与利用重点实验室,北京100081 [2]中国科学院石家庄农业现代化研究所,石家庄050021 [3]山东农业大学植物保护学院,泰安271018

出　　处：《中国生态农业学报》2003年第3期16-18,共3页Chinese Journal of Eco-Agriculture

基　　金：国家重点基础研究(973)发展规划项目(G2000016209);欧盟项目(ICA4-CT-2001-10069);"十五"国家科技攻关重大项目(2002BA516A01);"十五"中国科学院农业项目(NK十五-A-03)

摘　　要：以2种转Bt基因抗虫棉和1种常规棉上的棉蚜饲喂中华草蛉,试验研究Bt毒蛋白对捕食性天敌的影响结果表明,不同处理间中华草蛉幼虫和茧的死亡率以及成虫获得率无显著差异,用混合棉蚜饲喂的中华草蛉幼虫与饲喂“泗棉3号”和“GK-12”的相比发育历期显著缩短(P<0.01),与用“新棉99B”饲喂的相比其发育历期也明显缩短(P<0.05),而分别以“泗棉3号”、“GK-12”和“新棉99B”上的棉蚜单独饲喂的幼虫发育历期无显著差异。不同处理间中华草蛉茧期、茧重和成虫性比差异不显著,成虫产卵前期和产卵期也无明显差异。而饲喂“新棉99B”的单雌产卵量高于饲喂“泗棉3号”和“GK-12”,且成虫寿命延长。幼虫期饲喂“泗棉3号”、“GK-12”和“新棉99B”上的蚜虫成虫产卵的孵化率无显著差异,而饲喂混合棉蚜时其后代卵的孵化率明显降低。Effects of Bt toxin on the development and fecundity of Chrysopa sinica Tjeder fed on Aphis gossypii Glover reared on three cotton varieties (Simian-3,GK-12 and 99B) and on mixed Aphis gossypii were studied in laboratory. The results show that the mortalities of larva and cocoon, the percentage of adult achievement have no significant differences among these four treatments. When larva fed on mix aphides, its development time is significantly shorter than that fed on Simian-3 or GK-12 aphid, and also shorter than that fed on 99B aphid. There is no significant influence when Chrysopa sinica fed on either Simian-3 and GK-12 or 99B aphid. When Chrysopa sinica fed on 4 treatments aphides, there are no significant differences among their developmental time, cocoon weight, and sex ratio of adult. Aphis gossypii reared on Bt cotton have no detrimental effect on preovipositing and oviposition time of it. But Chrysoperla female adult fed on 99B and mixed aphides can lay significantly more eggs, and its lifespan is significantly longer than that fed on Simian-3 or GK-12. There is no phenomenal influence on percentage hatched eggs either their parental larva fed on Simian-3, GK-12 or 99B aphides. When the mixed aphides are given to the parental larva of Chrysoperla, the hatching rate is significantly lower than that fed on Simian-3, GK-12 or 99B aphid.

关 键 词：中华草蛉 发育 繁殖 转BT基因抗虫棉 捕食性天敌 棉蚜 BT毒蛋白 棉花害虫 

分 类 号：S435.622.1[农业科学—农业昆虫与害虫防治] S476.2[农业科学—植物保护]