七子花组培快繁技术研究初探  

Preliminary study on the tissue culture of Heptacodium miconioides Rehd.

作　　者：徐根娣[1] 刘鹏[1] 章振华[1] 张颖[1] 

机构地区：[1]浙江师范大学生命与环境科学学院,金华321004

出　　处：《中国生态农业学报》2003年第3期41-44,共4页Chinese Journal of Eco-Agriculture

基　　金：浙江省自然科学基金

摘　　要：对七子花幼叶诱导愈伤组织及愈伤组织诱导茎的培养基进行筛选,试验研究Mo、Mn及一些促进生根的植物生长激素对扦插枝生根的影响结果表明,愈伤组织的诱导以用嫩叶进行诱导的MS+0.5mg/kg2,4A-D+1mg/kgIBA+0.5mg/kgZT加1g/kg活性碳的培养基较好,茎的诱导以1/2MS+2mg/kg6-BA+0.2mg/kgNAA的培养基最佳,七子花扦插繁殖用6-BA处理成活率增加最明显。The media of tender leaves-induced callus and callus-induced buds were screened and the effects of Mo, Mn and some plant hormones on cutting production of Heptacodium miconioides were analyzed. The results show that tender leaves are more beneficial to callus inducement than buds and MS + 0.5mg/kg2,4-D+ 1mg/kgIBA+ 0.5mg/kgZT+ Ig/kg active carbon is the most advantageous medium to callus inducement; 1/2 MS+ 2mg/kg6-BA+ 0.2mg/kgNAA is the most profitable medium to buds induction; treatment of 6-BA is the most useful to cutting production.

关 键 词：七子花 组织培养 快繁技术 扦插繁殖 培养基 生物多样性保护 愈伤组织 观赏树种 

分 类 号：S68[农业科学—观赏园艺] Q949.781.2[农业科学—园艺学]