RAPD技术在中国对虾群体鉴别上的应用  

Application of RAPD technology for identification in three different stocks of Penaeus chinensis

作　　者：刘振辉[1] 孔杰[2] 刘萍[2] 邓景耀[2] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003 [2]中国水产科学院黄海水产研究所,山东青岛266071

出　　处：《海洋学报》2003年第4期67-71,共5页

基　　金：国家"973"计划资助项目(G1999012007)

摘　　要：采用RAPD技术对中国对虾(Penaeus chinensis)、中国黄海、渤海沿岸种群(H)朝鲜半岛西海岸种群(K)和即墨健康养殖群体(S)的DNA进行检测,41个随机引、物在3个群体间共筛选到了3个稳定的RAPD标记F-02_(540),OPN-13_(800)和OPN-20_(2200),可用于群体间的鉴别,研究将为中国对虾分子标记遗传育种提供依据。Three stocks (K, H and S) of Penaeus chinensis were analysed using 41 random oligonucleotide primers with 10 bases by RAPD method. K was from the spawning stock in the western coastal waters of Korea Peninsula; H was from the Huanghai (Yellow) Sea-Bohai Sea stock; S was from the health cultured stock in Jimo, Shandong Province. Three genetic markers which can identify the three stocks were got in this study. The study may provide the basic materials for marker assisted selection (MAS) of Penaeus chinensis.

关 键 词：RAPD 中国对虾 群体鉴别 养殖 标记遗传育种 DNA检测 

分 类 号：S966.12[农业科学—水产养殖]