异亚丙基莽草酸对血管内皮细胞释放前列环素的影响  

Effects of 3,4-oxo-isopropylidene-shikimic acid on the PGI_2 Release in Vascular Endothelial Cell

作　　者：马怡[1] 孙建宁[1] 徐秋萍[1] 郭亚健[1] 

机构地区：[1]北京中医药大学中药学院药理教研室,北京100102

出　　处：《北京中医药大学学报》2003年第4期25-27,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家科技部"十五"科技攻关项目 (No .2 0 0 1BA70 1A0 7-14 )

摘　　要：目的　研究异亚丙基莽草酸 (ISA)对体外培养的人脐静脉内皮细胞 (HUVEC)释放前列环素的影响。方法　用放射免疫法测定细胞培养液中前列环素代谢物 6 -酮 -前列腺素F1α(6 keto PGF1α)含量 ;噻唑蓝 (MTT)比色法检测细胞活性 ;硝酸还原酶法测定细胞培养液中NO水平。结果　异亚丙基莽草酸可促进正常的和H2 O2 损伤的HUVEC释放前列环素 ;ISA对正常的HUVEC增殖活性和NO的释放没有影响。we obseved the effect of 3,4 oxo isopropylidene shikimic acid (ISA) on the the PGI 2 release in human umbilical vein endothelial cells. Radioimmunoassay was used to assess 6 keto prostaglandin F 1α (6 keto PGF 1α ). Cell viability was assessed by MTT assay. The release of NO was assessed by colorimetry. The results showed that ISA increased the level of 6 keto PGF 1α in normal and H 2O 2 induced HUVEC. ISA had no effect on the viability and the NO release of the normal HUVEC So we conclude ISA incresed the PGI 2 release, which is related to the antithrombosis effect of ISA.

关 键 词：异亚丙基莽草酸 内皮细胞 6-酮-前列腺素F1Α 

分 类 号：R285.5[医药卫生—中药学]