HPLC法监测DL-丝氨酸的合成研究  

Monitoring a process for preparing DL-serine by HPLC

作　　者：傅莉[1] 刘信洪[1] 徐春艳[1] 薛传薪[1] 

机构地区：[1]北京化工大学理学院,北京100029

出　　处：《北京化工大学学报（自然科学版）》2003年第4期105-108,共4页Journal of Beijing University of Chemical Technology(Natural Science Edition)

摘　　要：采用HPLC方法 ,以OPA为柱前衍生化试剂 ,对常压下甘氨酸铜法合成DL 丝氨酸的过程进行了监控 ,通过改变加料方式及调节 pH值 ,提高了丝氨酸的含量 ,得出了常压下丝氨酸的最佳合成条件为 :n铜离子∶n甘氨酸∶n甲醛=1∶2 78∶973,pH =8 5～ 9 5 ,反应时间 1 5h ,丝氨酸最高质量浓度为 18 86 g/L。DL-serine was, from glycine and formaldehyde, prepared catalytically in an alkali medium at an atmosphere and the reaction mixture was analyzed by HPLC quantitatively. The reaction conditions, such as charging mode,molar ratio,pH value and so on, were examined. The optimal conditions are experimentally selected as follows: n _ copper ion ∶ n _ glycine ∶ n _ formaldehyde =1∶278∶973, pH=8.5～9.5, in the way of adding formaldehyde with an alkali medium. The reaction time is 1.5?h and the best yield of serine under these conditions is about 18.86?L/g.

关 键 词：甘氨酸 丝氨酸 HPLC α-羟甲基丝氨酸 

分 类 号：Q517[生物学—生物化学]