秋水仙素处理‘罗田甜柿’获得12倍体再生植株  

Regeneration of Dodecaploid Plants from in Vitro Leave of'Luotian Tianshi'Persimmon Treated with Colchicine

作　　者：谷晓峰[1] 罗正荣[1,2] 

机构地区：[1]华中农业大学园艺林学学院 [2]湖北民族学院园艺系,恩施445000

出　　处：《园艺学报》2003年第3期325-327,共3页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目 (30 0 70 5 2 9) ;教育部优秀青年教师资助计划项目 ;高等学校博士学科专项基金项目 (19990 0 40 0 1)

摘　　要：以‘罗田甜柿’试管苗离体叶片为材料 ,研究了秋水仙素浓度及处理时间对诱导染色体倍性变异的影响。结果表明 :0 3%秋水仙素处理 4～ 6d ,可诱导染色体加倍 ;与对照比较 ,再生变异植株的气孔孔径增大 ,密度降低 ,根尖染色体数 2n =12x =180 ,叶片DNA含量为对照的两倍。LⅠ、LⅡ、LⅢ细胞层均加倍的证据表明 ,获得的倍性变异株为同质 12倍体。A study of inducing polyploidy by treating'Luotian Tianshi'persimmon in vitro leaflet explants with colchicine solution was performed.The results indicated that mutated plantlets can be induced by treating the leaflet in 0.3% colchicines solution for 4-6 d.Stomata of the mutated plantlets were significantly larger than those of the normal plantlets,but the density of stomata were reduced significantly.The chromosome number of root-tip cell of mutated plantlets was 2n=12x=180,and nuclear DNA content of the mutated plantlets were twice as much as normal hexaploid plantlets.These results suggest that the ploidy of mutated plantlets was autododecaploid.

关 键 词：秋水仙素 '罗田甜柿’ 12倍体 再生植株 染色体 倍性变异 气孔孔径 密度 染色体数 

分 类 号：S665.2[农业科学—果树学]