HCMV先天性感染胎鼠脑组织中iNOS基因的表达  

Expression of inducible nitric oxide synthase (iNOS) in fetal mouse cerebral cortex following congenitally infected with human cytomegalovirus

作　　者：余莉[1] 李艳秋[1] 张文艳[1] 方草晖[1] 类延花[1] 吴建军[1] 金晶[1] 王明丽[1] 

机构地区：[1]安徽医科大学微生物教研室,合肥230032

出　　处：《安徽医科大学学报》2003年第3期175-178,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省生物医药重大科技专项 (编号 :0 13 0 3 0 0 3 ) ;教育部科学技术研究重点项目 (编号 :0 10 5 2 )

摘　　要：目的　研究人巨细胞病毒 (HCMV)先天性感染小鼠及在不同剂量诱导型一氧化氮合酶 (iNOS)抑制剂和促进剂的干预下脑组织中的iNOS基因表达情况。方法　将 6～ 8周龄BALB/c小鼠分为 6组 ,每组 6只 ,♀♂各半。A组腹腔注射HCMV 1 0ml,B组、C组腹腔注射HCMV 1 0ml后2d ,分别每天腹腔注射氨基胍 (AG) 2 0 0mg/kg、2 0mg/kg ,D组、E组腹腔注射HCMV 1 0ml后 2d ,分别每天腹腔注射左旋精氨酸 (L Arg) 30 0mg/kg、30mg/kg ,F组为DMEM对照组 ,每天每只 0 2ml。持续 17d。以上各组在孕鼠受孕第19天 ,剖腹取胎鼠 ,观察各组胎鼠生长发育及体重 ,有无皮下出血、淤血等情况 ,同时取胎鼠脑组织 ,作以下检测 :①硝酸还原酶法测定脑组织匀浆中NO代谢产物亚硝酸盐的含量。②RT PCR测iNOSmRNA的转录。结果　HCMV感染各组孕鼠在孕 19d ,其体重明显低于对照组 ,有部分胎鼠发育迟缓并可见明显的出血现象。感染各组胎鼠脑组织匀浆NO代谢产物明显高于DMEM对照组 (P <0 0 0 1) ,以B组尤为明显 ,A、C、E组间无差异显著性。RT PCR在感染各组均扩增出iNOSmRNA特异性条带表达 ,B组信号量最高 ,D组信号最弱。DMEM对照组未检测到iNOS特异性条带。结论　HCMV先天性感染可刺激胎鼠脑组织iNOS的表达 ,其表达产物NO可能参与了脑组织病理损伤?Objective The study aimed at investigating expression of iNOS in fetal mouse cerebral cortex following congenitally infected with human cytomegalovirus. Methods 36 6～8 week old BALB/c mice were divided into 6 groups,with 3 femal and 3 male mice in each group. Group A was subjected to the intraperitoneal injection of 100 TCID_ 50 1.0 ml. AG was injected into the intraperitoneum of group B (200 mg/kg) and group C(20 mg/kg) respectively after 2 d of HCMV administration. Group D and group E were given intraperitoneal injection of 300 mg/kg and 30 mg/kg L-Arg respectively after 2 d of HCMV infection. Group F was used as normal control group. On the 19th gestation-day and just before delivery,the brain of fetuses were collected by laparotomy. The weight of each fetal was measured and teratism observed. Brain homogenate level of NO was determined by NO enzyme Kit. iNOS mRNA expression in fetal brain was detected with RT-PCR. Results The weight of HCMV infection group was lower than that of control group. NO level of fetal brain homogenate in infection group was significantly higher than that in the control group(P<0.001). iNOS mRNA was expressed in HCMV infection fetal mice but not in control group. Conclusion Congenital HCMV infection stimulats iNOS mRNA expression in the brain of fetal mouse,and NO,the prodution of iNOS may participate in the brain damage caused by congenital HCMV infection.

关 键 词：人巨细胞病毒感染 胎鼠 脑组织 INOS基因 基因表达 

分 类 号：R512.99[医药卫生—内科学]