CuZn-SOD乳酸-羟乙酸共聚物缓释微球的制备及体外、体内释放研究  

Preparation and In Vitro and In Vivo Release of Copper and Zinc Superox-ide Dismutase Encapsulated into PLGA Microspheres

作　　者：刘玲[1] 葛宇[2] 袁勤生[2] 

机构地区：[1]南京医科大学公共卫生学院,南京210029 [2]华东理工大学生物化学研究所生物反应器工程国家重点实验室,上海200237

出　　处：《中国生物制品学杂志》2003年第4期208-211,共4页Chinese Journal of Biologicals

摘　　要：目的 探讨CuZn-SOD包裹入乙酸-羟乙酸共聚物制备的药物微球的缓释效果。方法 采用复乳法制备CuZn-SOD乙酸-羟乙酸共聚物(PLGA)药物微球,分别用恒温摇瓶法和ELISA法检测体外和体内的释放效果。结果 有机相/分散相体积比、内水相体积、蛋白质浓度等因素影响微球的性质,由于聚合物的吸附作用,CuZn-SOD的体外释放呈明显的两段释放,释放不完全;体内释放显示微球制剂有一定的缓释效果。结论 该微球有比较好的缓释作用。Objective To explore the control-release of microspheres prepared by the encapsulation of copper and zinc superoxide dismutase(CuZn-SOD) into PLGA. Methods CuZn-SOD was encapsulated into PLGA microspheres by double emulsion method.The in vitro and in vivo release of CuZn-SOD from microspheres were detected by incubation method and ELISA respectively. Results The nature of microspheres were influenced by the volume ratio of organic phase to disperse phase, volume of intra-aqueous phase, protein concentration,and so on. Because of the adsorption of polymer, CuZn-SoD released by two stages and incompletely in vitro. However,it showed a control-release in vivo.Conclusion The microspheres showed long control-release.

关 键 词：乳酸-羟乙酸共聚物 缓释微球 释放 重组人铜锌超氧化物歧化酶 蛋白质药物 

分 类 号：TQ464.7[化学工程—制药化工]