山东沿海21种海洋无脊椎动物抗肿瘤活性初步筛选  

ANTICANCER ACTIVITY OF 21 MARINE INVERTEBRATE SPECIES

作　　者：张立新[1] 范晓[1] 李宪璀[1] 

机构地区：[1]中国科学院海洋研究所,青岛266071

出　　处：《海洋科学》2003年第7期63-67,共5页Marine Sciences

基　　金：国家863计划资助项目2001AA620403号

摘　　要：采用四氮唑盐比色法(MTT)测定了21种青岛及其附近海域常见无脊椎动物94％乙醇粗提物对人白血病细胞HK-60和人肺腺癌细胞A-549的抑制率,同时测定了海筒螅Tubuariamarina Torrey等动物不同极性溶剂萃取物的活性。发现海燕Asterina pectinifera的正丁醇萃取物、海筒熄的乙酸乙酯萃取物、红条毛肤石鳖Acanthochiton rubrolineatus的94％的乙醇提取物对HL-60的抑制作用很强,在浓度为0.063mg/mL时抑制率分别为96.7％、63.9％、50.5％;海筒螅的乙酸乙酯萃取物对A-549的抑制率较高,浓度为0.063mg/mL时的抑制率可以达到65.4％。The anticancer activity of 21 marine invertebrate species which are common near Qingdao seashore was determined by MIT method. It was found that the n-butanol part of Asterina pectinifera, the acetic ether part of Tubuaria marina Torrey, 94% ethanol extract of Acanthochiton rubrolineatus have much stronger inhibition rates on tumor cell HL60, which are 96. 7%, 63.9% and 50. 5% respectively in the concentration of 0.063mg/mL. The inhibition rate of the acetic ether part of Tubuaria marina Torrey on the tumor cell A-549 is 65. 4 % in the concentration of 0. 063 mg/mL.

关 键 词：山东 海洋 无脊椎动物 抗肿瘤活性 四氮唑盐比色法 

分 类 号：R282.77[医药卫生—中药学] R730.1[医药卫生—中医学]