人生长激素基因在小鼠体内的高效表达  

High expression of human growth hormone gene in mouse

作　　者：张文[1] 宋向凤[2] 王辉[2] 

机构地区：[1]新乡医学院生物化学教研室,河南新乡453003 [2]新乡医学院微生物学与免疫学教研室,河南新乡453003

出　　处：《新乡医学院学报》2003年第4期232-233,236,共3页Journal of Xinxiang Medical University

基　　金：河南省自然科学基金资助项目 (0 0 4 0 2 14 0 0 )

摘　　要：目的　观察人生长激素基因在小鼠体内的表达。方法　将构建的表达质粒直接注射于小鼠的股四头肌内 ,通过免疫组化和ELISA方法检测注射部位和血清中生长激素(GH)的表达情况。结果　免疫组化显示在注射部位有GH的表达 ,在血清中GH的浓度从d5开始即有表达 ,在d90仍有表达。结论　人生长激素基因通过直接注射法可以在小鼠体内有较长时间的表达 ,这为临床上基因治疗提供了新的途径。Objective To investigate the expression of human growth hormone(hGH) gene in mouse.Methods After injection of the plasmid pcDNA3-hGH,the expression of hGH was assayed by immunohistochemistry and ELISA.Results The expressions of hGH were observed in injection site and the concentration of hGH in serum was detected from the fifth day to the ninetieth day. Conclusion The study suggests that long-term GH gene expression may be obtained in mouse by directly injecting plasmid. This could provide a new genetic therapy for growth hormone deficiency.

关 键 词：人生长激素 基因治疗 小鼠 

分 类 号：Q575.11[生物学—生物化学]