AFLP分析小豆种(Vigna angularis)内遗传多样性  

Genetic Diversity in Vigna angularis Revealed by AFLP Analysis

作　　者：宗绪晓[1] D Vaughan A Kaga N Tomooka 王新望 关建平[1] 王述民[1] 

机构地区：[1]中国农业科学院作物品种资源研究所,北京100081 [2]日本农业生物资源研究所

出　　处：《作物学报》2003年第4期562-568,共7页Acta Agronomica Sinica

基　　金：IPGRI与JIRCAS联合资助的中;日;韩;朝国际合作项目 :"东亚小豆综合种群遗传多样性分析"( 1999~ 2 0 0 2 )

摘　　要：利用 12对AFLP引物 ,对来自于世界 6个国家的 14 6份小豆 (Vignaangularis)栽培变种 (var.angularis)和野生变种 (var.nipponensis)种质的基因组DNA进行扩增 ,得到 5 80条清晰的显带 ,其中 313(5 3 93% )条呈多态性 ,平均每对AFLP引物得到 2 6 0 8条多态性带 ;平均遗传距离 0 35 ,变异幅度为 0 0 0～ 0 87。利用AFLP多态性数据进行的Jaccard’s遗传距离聚类分析绘制的聚类图 ,可将其中的 14 3份种质相互区分开 ,并将其中的 14 5份小豆资源划分成 8个明显不同组群 ,显示小豆种内存在足够的遗传多样性用于资源材料的准确鉴别与分类。 8个组群的遗传多样性表现出十分明显的地域相关性 ,以及遗传类型趋同性。通过各组群内和组群间的遗传距离比较 ,发现世界小豆主要栽培资源以及日本野生、半野生资源中蓄积的遗传多样性较匮乏 ,而储藏于中国野生小豆资源、喜马拉雅地区栽培和野生资源中的遗传多样性较丰富。A representative set of 146 azuki ( Vigna angularis var. angularis , and var. nipponensis ) germplasm from worldwide geographical regions, was used for AFLP analysis with 12 informative primer pairs. 580 unambiguous bands were created, 313 of which were polymorphic. One pair of AFLP primers has detected a mean of 26.08 polymorphic bands. All the 313 polymorphic bands were used to assess genetic distance among 146 azuki accessions to form the genetic distance matrix, by using Jaccard's method. The mean genetic distance among materials was 0.35 with a range of 0.00 to 0.87 according to the matrix. Eight clusters were detected by using UPGMAM cluster analysis, based on the genetic distance matrix. According to the dentrogram formed by cluster analysis, 143 from the whole materials were distinctly clarified, revealed enough genetic diversity for identification and classification of accessions within Vigna angularis . Eight clusters are clearly corresponded to the geographic origin of accessions. Comparing the genetic distances within and between clusters, the shortage of genetic diversity in the main cultivated accessions, as well as wild and weedy accessions from Japan is revealed; Whilst the broader genetic diversity is shown in the wild accessions else where and in the cultivated accessions from Himalayan area.

关 键 词：AFLP分析 小豆 Vigna-angularis 遗传多样性 栽培变种 野生变种 

分 类 号：S521[农业科学—作物学]