dsDNA芯片的DNA结合蛋白质计算机模拟系统  

A COMPUTER SIMULATION SYSTEM OF DNA-BINDING PROTEIN EXPERIMENT BASED ON dsDNA MICROARRAY

作　　者：谢建明[1] 白云飞[1] 钱璐璐[1] 崔磊[1] 孙啸[1] 陆祖宏[1] 

机构地区：[1]东南大学吴健雄实验室,江苏南京210096

出　　处：《生物物理学报》2003年第2期156-160,共5页Acta Biophysica Sinica

基　　金：国家自然科学基金创新群体基金(60121101)

摘　　要：dsDNA芯片(double-stranded DNA microarray)是用于DNA结合蛋白质表达研究的新技术,应用一种新的dsDNA芯片制作方法,研究了dsDNA探针设计,开发了相应的计算机模拟系统——DBP软件。可实现芯片上dsDNA探针的选取以及DNA结合蛋白质检测的实验过程模拟,功能包括模拟酶切、模拟电泳、模拟杂交等实验环节。DBP系统包括了可以不断完善的DNA结合蛋白质数据库。对研究dsDNA 芯片,检测和分析在生物体中DNA结合蛋白质的表达谱,揭示细胞的分化和凋亡、信号转导、DNA转录调控的分子机制有重要意义。DsDNA(double-stranded DNA) microarray, as a novel high-throughout technique, get a start in the field of DNA-binding protein research. It studied a dsDNA probe designing method for a new fabrication technology of the dsDNA microarray. Then a computer software named 'DBP' was intro-duced, which simulated the procedures of DNA-binding protein experiment based on dsDNA microarray and included DNA digestion using restriction enzymes, electrophoresis, hybridization and data manage-ment. Using DBP software, it can design dsDNA probes give advice on experiment planning and predic-tive results of a DNA-binding protein experiment.

关 键 词：dsDNA芯片 DNA结合蛋白质 计算机模拟系统 探针设计 生物信息学 

分 类 号：Q617[生物学—生物物理学] Q71