高效液相色谱法测定埃普利诺菌素注射液的含量  

Determination of eprinomectin in injection by high performance liquid chromatography

作　　者：潘保良[1] 汪明[1] 王玉万 潘贞德 薛彦 

机构地区：[1]中国农业大学动物医学院,北京100094 [2]中农大生物技术股份有限公司,北京100094

出　　处：《中国兽药杂志》2003年第7期28-30,共3页Chinese Journal of Veterinary Drug

摘　　要：　本文描述了用高效液相色谱法(HPLC)测定埃普利诺菌素注射液含量的方法研究。色谱条件:流动相为甲醇∶水(90∶10),流速1.0mL/min,进样量50μL,柱温为室温,检测波长245nm。样品回收率为(98.72±0.28)%。在5～200μg/mL浓度范围内,检测样品的中间精密度:批内变异系数为0.54%～0.80%,批间变异系数为0.28%～1.06%。本方法可用于埃普利诺菌素注射液的含量检测。Determination of eprinomectin in injection by high performance liquid chromatography was developed. The column temperature was room temperature. The mobile phase, consisting of methanolwater(90+10, V/V), was pumped at 1 mL/min. The detector was fixed at 245 nm. The injection volume was 50 μL. Recovery of detected sample was (98.72±0.28)%. In the range of concentration from 5 to 200 μg/mL, the interassay precision of the method expressed as the CV was 0.28%～1.06% and the intraassay precision was 0.54%～0.80%. This method is suitable for the determination of eprinomectin in injection.

关 键 词：埃普利诺菌素注射液 含量测定 高效液相色谱法 变异系数 驱虫药剂 

分 类 号：S859.795[农业科学—临床兽医学]