酶联免疫吸附间接法检测抗子宫内膜抗体及其临床应用  

THE USE OF THE INDIRECT ELISA IN THE DETERMINATION OF ANTIENDOME- TRIAL ANTIBODIES AND ITS CLINICAL APPLICATION

作　　者：黄邱朝[1] 庄恭南 武建国[1] 刘琦[2] 

机构地区：[1]南京军区南京总医院临床免疫科 [2]南京军区南京总医院妇产科

出　　处：《解放军医学杂志》1992年第1期29-30,共2页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金

摘　　要：将子宫内膜用胶原酶消化后依序过250μm、40μm筛网,获内膜腺体,匀浆后上清流穿抗人血浆亲和层析柱,即得纯化人子宫内膜抗原。用此抗原建立了检测抗子宫内膜抗体的酶联免疫吸附检测(ELISA)间接法,不孕妇女(n=129)与流产妇女(n=113)阳性率分别为43.4％和37.1％;男性对照(n=43)为0,正常孕妇(n=52)为3.8％,中、老年妇女(n=120)为6.7％。The endometrial antigens from human endometrium were purified by collagenase (Type I), 250um, 40um, sieves and affinity chromatography. Indirect ELISA was used to detect antiendometrial antibodies (EMAb). The findings showed that the positive rates of EMAb in this assay were 43.4% (infertile, n=129), 37.1% (abortion, n=113), 6.7% (middle and old man, n = 120), 3.8% (pregnant woman, n = 52) and 0% (male, n = 43), respectively.

关 键 词：不育症 女性 抗体 ELISA EMAB 

分 类 号：R711.603[医药卫生—妇产科学]