铅对孕鼠脑垂体卵巢胎盘及人胎盘绒毛毒性作用的研究  

LEAD TOXICITY ON PITUITARY, OVARY AND PLACENTA OF PREGNANT RAT AND HUMAN CHORIONIC VILLI OF PLACENTA

作　　者：喻凯[1] 童夙明[1] 

机构地区：[1]上海医科大学组织学胚胎学教研室,上海200032

出　　处：《解剖学报》1992年第4期431-436,共6页Acta Anatomica Sinica

摘　　要：用妊娠第7d大鼠,实验Ⅰ和Ⅱ组分别每日灌服硝酸铅100mg/kg和200mg/kg,连续5d。于妊娠第12d检查,实验Ⅰ组血铅浓度27.90±8.47μg/dl,黄体和胎盘可出现轻度损害。实验Ⅱ组孕鼠血铅浓度48.77±16.15μg/dl,相当于人亚中毒血铅水平,黄体和胎盘的光镜或超微结构损害严重,黄体3β-羟甾脱氢酶为对照组的1/20,31.69％的胚胎被吸收。垂体前叶细胞未见明显变化。人胎盘绒毛体外培养,实验组的培养液内分别加入硝酸铅0.5μg/ml、2.5μg/ml、5μg/ml,培养3～4d后进行形态学观察。培养液中含铅量2.5μg/ml以上者,绒毛的光镜及超微结构均有明显损害,培养液中hCG含量也明显减少。本文结果提示,铅使孕鼠的黄体、胎盘及胎鼠毒性损害明显,对人胎盘绒毛也有直接毒害作用。Female rats were fed with lead acetate 100mg/kg/day(group Ⅰ) or 200 mg/kg/day (group Ⅱ) for 5 days, at the day 7 to 11 of gestation prior to killing. The results shown that the mean level of blood lead in rats of group I was 27.90±8.47μg/dl, and corpus luteum or placenta were injured lightly. In the group Ⅱ, the level of blood lead reached 48.77±16.15μg/dl which correspond to human subacute intoxication, the structure of corpus luteum and placenta were injured prominently, and the activity of 3-hydroxysteroid dehydrogenase is lower than that of control group, about 1/20 of latter. The number of embryos in group Ⅱ were absorbed about 31.69%. However, the gonadotropic cells of pituitary did not affected prominently after treatment with lead. The human chorionic villi of placenta were cultured in medium with lead acetate 0.51μg/ml, 2.5μg/ml, 5μg/ml respectively, for 3 to 4 days. The struture of chorionic villi were damaged obviously after culture with lead more than 2.51μg/ml, and the concentration of hCG in culture medium was decreased. The results indicated that lead affected the corpus luteum, placenta and embryo, and exerted direct action on human trophoblast.

关 键 词：硝酸铅 脑垂体 黄体 人胎盘绒毛 

分 类 号：R992[医药卫生—毒理学]