胶体金法研究胎儿胸腺上皮网状细胞的细胞学观察  

AN OBSERVATION ON THE CYTOLOGICAL STUDY OF FETUS THYMUS RETICULAR CELLS WITH THE COLLOIDAL GOLD METHOD

作　　者：戴洁[1] 薄爱华[1] 王淑强[1] 夏苓[1] 陈德慧[2] 张贺秋[2] 张竞时[1] 祁晓莉[1] 李海峰[1] 左东升[1] 何丁[1] 

机构地区：[1]河北张家口医学院,张家口075000 [2]解放军军事医学科学院基础三所

出　　处：《解剖学杂志》1992年第2期95-98,共4页Chinese Journal of Anatomy

摘　　要：为了探讨胎儿胸腺上皮网状细胞发育过程的结构特点,用胶体金免疫组化法及电镜观察60例胎龄12～42周胸腺。结果表明:不同类型的上皮网状细胞中间丝有多寡之别并含有不同类型角蛋白;中间丝多者细胞器相对减少。角蛋白阳性反应细胞体积随胎龄增长而增大,但阳性细胞出现率与胎龄关系不大。角蛋白阴性反应细胞电镜下可见丰富的内质网、线粒体及核糖体,提示该细胞主要有分泌胸腺激素功能。In order to study the development and the ultrastructure of the thymic reticular cells of the fetus, we observed 60 human fetal thymuses aged 12-42 weeks of gestation with the immunogold silver staining method and the transmission electron microscopy. The results showed that in various reticular cells there appeared various . numbers of the intermediate filament and various types of the keratin in the intermediate filament.The cytoplasm with more intermediate filament contained relatively less organelles.Furthermore, the size of the keratin positive reticular cells grew with aging.The incidence of the positive cells, however, related little to the developmeni of the embryo. It's also discovered that the keraiin negaiive reticular cells were rich in rough endoplasmic reticulum, mitochondria and free ribosomes. And this suggested that its function was mainly to secrete thymic hormone.

关 键 词：胎儿胸腺 上皮网状细胞 免疫组化 

分 类 号：R322.53[医药卫生—人体解剖和组织胚胎学]