谷氨酰胺对饲喂生大豆仔猪小肠结构和功能的影响  

Effect of Dietary Glutamine Supplementation on Intestinal Structure and Functions in Piglets Fed on Diets Containing Raw Soybean

作　　者：张军民[1] 高振川[1] 王连娣[1] 姜云侠[1] 张琪[1] 

机构地区：[1]中国农业科学院畜牧研究所,北京100094

出　　处：《畜牧兽医学报》2003年第4期356-361,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：选18头28日龄断奶体重相近的大长北公仔猪,随机分为3组,每组6头于代谢笼中进行试验。按等氮等能原则平衡各组日粮,基础日粮含5%的生大豆,试验Ⅰ组添加1%的谷氨酰胺、试验Ⅱ组添加2%的谷氨酰胺,49日龄屠宰所有仔猪以研究谷氨酰胺对仔猪小肠结构和功能的影响。结果表明试验Ⅱ组仔猪十二指肠粘膜中的蛋白质含量显著高于试验Ⅰ组和对照组。而空肠和回肠中蛋白质两试验组都高于对照组,但无显著差异;各组间肠道DNA含量无显著差异。两试验组仔猪回肠粘膜重量显著低于对照组,而十二指肠和空肠各组间无明显差异。试验Ⅰ组仔猪十二指肠绒毛高度极显著高于对照组,也显著高于试验Ⅱ组,试验Ⅱ组仔猪十二指肠绒毛高度显著高于对照组;试验Ⅰ组仔猪空肠中段绒毛高度也显著高于对照组。而空肠前段和空肠后段各组间无显著差异。不同肠段的粘膜厚度也无显著差异。试验Ⅰ组空肠中乳糖酶活性极显著低于对照组,而肠道亮氨酰氨基肽酶活性各组间无显著的差异。试验表明了谷氨酰胺可以缓解因饲喂生大豆造成仔猪肠道的损伤。Eighteen piglets weaned at the 28 d of age were randomly assigned to one of three groups (0%,1%,2%glutamine supplementation).The basal diet contained 5% raw soybean.The diets were calculated to be isonitrogenous and isoenergetic.The piglets were killed at 49 d of age.The results showed that dietary glutamine supplementation increased the content of duodenum protein.In treatment Ⅱ,duodunum protein content were significantly higher than that in treatment Ⅰ and control.In jejunum and ileum,there was a trend for protein content to rise with increasing glutamine dosage.There were no significant differences in intestinal DNA content.In treatment Ⅰ and Ⅱ,the ileum mucosal weight were significantly lower than that in control,but there were no significant differences in duodenum and jejunum mucosal weight.Dietary glutamine supplementation in creased the villus height in duodenum and middle jejunum;In treatment Ⅰ and Ⅱ,the duodenum villus height were significantly higher than that in control.The jejunum villus height in treatment Ⅰ was significantly higher than that in control.There were no significant differences of villus height in proximal and distal jejunum.The mucosal thickness were unchanged in control and treatment.There were no significant differences in activities of lactase and leucylaminopeptidase.The results show that dietary glutamine supplementation had protective effect on intestinal injury in piglets fed on diets containing raw soybean.

关 键 词：谷氨酰胺 饲喂效果 生大豆 仔猪 小肠 结构功能 影响因素 

分 类 号：S828[农业科学—畜牧学]