不同来源肽消失规律的体外法研究  

Studies on the Disappearance Patterns of Different Peptides in Vitro

作　　者：程茂基[1] 卢德勋[2] 蒋克纯[1] 王洪荣[2] 王岗[2] 赵秀英[2] 张海鹰[2] 郭文华[2] 

机构地区：[1]安徽农业大学畜牧学院,合肥230036 [2]内蒙古畜牧科学院,呼和浩特010018

出　　处：《畜牧兽医学报》2003年第4期362-367,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目资助 (39860059)

摘　　要：利用体外瘤胃微生物批次培养研究了大豆肽、玉米肽和瘤胃液肽的消失规律。结果表明:随着培养时间的推移,三种肽的消失量与消失率均显著增加(P<0 01,P<0 05),而消失速率却显著降低(P<0 01);当培养液中肽含量增加时,三种肽的消失量随之增加(P<0 01,P<0 05),消失速率也显著提高(P<0 01,P<0 05),而消失率却显著下降(P<0 05)。对于不同肽源来说,瘤胃液肽的消失量和消失率以及消失速率显著高于大豆肽(P<0 05)和玉米肽(P<0 01);大豆肽的消失量和消失率以及消失速率显著高于玉米肽(P<0 05),并且在培养中后期彼此差异逐渐变小(P>0 05)。肽的来源和结构不同,其消失规律也有异。The disappearance patterns of different peptides by rumen microorganisms were studied in vitro.The results showed that the disappearance amount and proportion of peptides were increased significantly(P<001,P<005) with incubation time,but the disappearance rates of peptides was decreased significantly (P<001).When peptides concentration was increased in the culture media,the disappearance amount and rates of peptides were increased significantly (P<001,P<005),but their disappearance proportion was decreased significantly (P<005).The disappearance amount,proportion and rate of rumen liquid peptides were higher than those of soybean protein peptide(P<005) and corn protein peptide(P<001).The disappearance amount,proportion and rate of soybean protein peptides were higher than those of corn protein peptide(P<005).The results showed that the disappearance patterns of peptides would be different because they were from different proteins and remained different compositions.

关 键 词：肽 消失规律 体外法 日粮 饲养管理 样品预处理 

分 类 号：S816[农业科学—饲料科学]