细胞粘附位点RGD三肽脂质体的制备及表征  

Preparation and Characterization of Liposome of RGD Tripeptide as Cell Recognition Site

作　　者：杨彤[1] 周云鋆 王娜[1] 徐力[1] 吴晓霞[1] 张学忠[1] 

机构地区：[1]吉林大学分子酶学工程教育部重点实验室,长春130023

出　　处：《吉林大学学报（理学版）》2003年第3期356-360,共5页Journal of Jilin University:Science Edition

基　　金：国家"十五"科技攻关计划基金(批准号:2001BA960C).

摘　　要：对RGD(Arg-Gly-Asp)三肽脂质体载药剂型进行研究,确定了薄膜超声法制备脂质体的路线.电子显微镜观察表明,脂质体粒径均匀,为100nm左右,属于小单室脂质体.脂质体中RGD量为2.4～2.5mg/mL,包封率达到70%.脂质体稳定性较好,而且具备pH5.4～6.0的靶向性,可能成为具有较好肿瘤细胞靶向性的脂质体制剂.In this paper, RGD(ArgGlyAsp) liposome formulation as a drug carrier was conducted. A filmultrasonic technique was chosen for preparation of liposome. The observation by electron microscope indicated that the particle diameter of liposome was basically homogenous, being about 100 nm and small vesicles. The content of RGD in liposome is 2.4～2.5 mg/mL with the entrapment efficiency of 70%. RGD liposome prepared by the method used has not only better stability, but also target tropism at pH 5.4～6.0. Therefore, the prepared liposome here may become a kind of RGD formulation with better target tropism to tumor.

关 键 词：抗肿瘤转移药物 RGD三肽 脂质体 细胞粘附位点 薄膜超声法 制备 性质表征 靶向药物 

分 类 号：R979.1[医药卫生—药品] TQ464.7[医药卫生—药学]