抗角蛋白自身抗体对培养角质形成细胞产生IL-6和IL-8的影响  

Effects of antikeratin auto antibody on the production of IL-6 and IL-8 by cultured keratinocytes

作　　者：张衍国[1] 刘玉峰[1] 武彩霞[1] 赵小东[1] 李巍[1] 付萌[1] 

机构地区：[1]第四军医大学西京医院全军皮肤性病中心,陕西西安710032

出　　处：《西北国防医学杂志》2003年第4期283-285,共3页Medical Journal of National Defending Forces in Northwest China

摘　　要：目的 :研究抗角蛋白自身抗体 (AKautoAb)对培养角质形成细胞 (KC)产生细胞因子的影响。方法 :以IL - 1 β刺激无血清培养的KC及AKautoAb处理后的培养KC ,用MH6 0细胞活性法检测培养上清中IL - 6的产生 ,用ELISA检测培养上清中IL -8的产生。结果 :IL - 1 β可刺激KC产生IL - 6和IL - 8(P <0 .0 1 ) ,并有IL - 1 β浓度依赖性 ;AKautoAb对培养的KC产生IL -6和IL - 8有明显抑制作用 (P <0 .0 1 )。结论 :AKautoAb对培养KC产生细胞因子有明显抑制作用 。Objective: To investigate the effects of AK auto Ab on the production of inflammatory factors by cultured keratinocytes. Methods: Keratinocytes were cultured in serum-free keratinocyte culture medium(KC-SFM), and then, cultured keratinocytes and AK auto Ab-pretreated cultured keratinocytes were stimulated with IL-1β. IL-6 and IL-8 in supernatant were assessed with MH60 lines proliferation assay or ELISA,respectively. Results: IL-1β stimulated keratinocytes to secrete IL-6 and IL-8 in a dose-dependent fashion( P <0.01);AK auto Ab inhibited the production of IL-6 and IL-8 by keratinocytes stimulated by IL-1β( P <0.01). Conclusion: AK auto Ab is able to significantly inhibit the production of inflammatory factors by cultured keratinocytes. This may be the main mechanism which contribute to the restore of dermatitis such as psoriasis.

关 键 词：角蛋白 自身抗体 角质形成细胞 白细胞介素6 白细胞介素8 

分 类 号：R751[医药卫生—皮肤病学与性病学]