氯化铬和重铬酸钾毒性的比较研究  

COMPARATIVE STUDY ON TOXICITIES OF POTASSIUM DICHROMATE AND CHRO MIUM CHLORIDE

作　　者：马华智[1] 盛和章[1] 秦爱平[1] 施畅[1] 李永涛[1] 廖明阳[1] 

机构地区：[1]军事医学科学院毒物药物研究所六所四室,北京100850

出　　处：《癌变．畸变．突变》2003年第3期171-173,共3页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的 :比较氯化铬和重铬酸钾两种价态铬化合物的细胞毒性、致突变性及急性毒性。 方法 :CHL细胞采用MTT比色法 ,沙门氏菌诱变性试验和小鼠急性毒性试验。 结果 :经MTT比色法测定 ,氯化铬和重铬酸钾对CHL细胞半数抑制剂量(IC50)分别为33.80mmol/L和8.02μmol/L,对小鼠口服急性毒性LD50 分别为2143.3和171.0mg/kg,表明重铬酸钾的细胞毒性和急性毒性均明显大于氯化铬 ,重铬酸钾诱发沙门氏菌回变菌落数比阴性对照增加2倍以上而氯化铬无明显增加。 结论 :重铬酸钾的细胞毒性和急性毒性均大于氯化铬 ,重铬酸钾对沙门氏菌具有致突变性 。Purpose: To compare the cycotoxixity,mutagenncity and mouse acute toxicity between Chromium chloride and Potassium di chromate. Methods: MTT method for determition of CHL Cell IC 5 0, Salmonella mutagenicity test, and mouse acute toxicity test were used. Results: The CHL cell IC 50 of chromium chloride and potassium dichromate were 33.80 mmol / L and 8.02 μmol / L,r espectively; the LD 50 of chromium chloride and potassium dichro mate in mouse acute toxicity test were 2 143.3 and 171.0 m g / kg,respectively. Potassium dichromate meet the positive respond standards of Salmonella mutagenicity test whereas chromium chlor ide showed a negative results. Conclusion: Both the cytotoxicity and mouse acute toxicity of potassium dichromate was larger than chromium chloride. Potassium dichromate was mutagennic to Salomamella strains,but chromium chloride was not.

关 键 词：重铬酸钾 氯化铬 MTT 沙门氏菌诱变性试验 

分 类 号：R994[医药卫生—毒理学]