模拟失重对大鼠比目鱼肌梭内肌纤维肌球蛋白重链表达的影响(英文)  

Effects of Simulated Weightlessness on Myosin Heavy Chain Expression of Soleus Intrafusal Muscle Fibers in Rats

作　　者：唐斌 樊小力[1] 吴苏娣 

机构地区：[1]西安交通大学医学院生理学教研室,陕西西安710061

出　　处：《航天医学与医学工程》2003年第4期235-238,共4页Space Medicine & Medical Engineering

基　　金：SupportedbytheNationalNaturalScienceFoundation (3 0 0 70 83 0 )

摘　　要：目的检查模拟失重对大鼠比目鱼肌梭内肌纤维肌球蛋白重链表达的影响 ,探讨模拟失重所致肌梭退变的机制。方法采用免疫过氧化酶反应ABC法观察尾悬吊 7d ,1 4d与对照组大鼠比目鱼肌梭内肌纤维对特异性抗小鸡肌球蛋白重链慢紧张型 (ALD5 8)和快缩型 (MF30 )单克隆抗体表达的差别。结果梭外肌纤维不与ALD5 8和MF30起反应。这两种抗体仅与梭内肌纤维反应。核袋 1和核袋 2纤维对ALD5 8抗体起反应。核袋 2和核链纤维对MF30抗体起反应。尾悬吊 7d后 ,核袋 1和核袋 2纤维对ALD5 8的免疫反应性降低 ,而核袋 2和核链纤维对MF30免疫反应性增强。尾悬吊 1 4d后 ,核袋 1和核袋 2纤维对ALD5 8的免疫反应性明显降低 ,甚至消失。而所有的梭内肌纤维对MF30都起免疫反应 ,并且明显增强。Objective To examine the myosin heavy chain expression in intrafusal fibers in rat soleus muscle before and after simulated weightlessness and to analyse the mechanism involved in muscle spindle deterioration during simulated weightlessness. Method The rats were divided into 3 groups: 7 d, 14 d tail suspension group and control group. Control and experimental spindles were examined using monoclonal antibodies specific for myosin heavy chains of slow tonic (ALD58) and fast twitch (MF30) chicken muscles by the immunoperoxidase reaction utilizing the ABC (avidin biotin complex) method. Result The extrafusal muscle fibers did not exhibit immunoreactivity to ALD58 and MF30. Only intrafusal fibers bound these antibodies. The bag 1 fiber and bag 2 fiber reacted to ALD58. The bag 2 fiber and chain fibers stained with MF30. After 7d suspension the immunoreactivity of the bag 1 fiber and bag 2 fiber to ALD58 decreased, while the immunoreactivity of the bag 2 fiber and chain fibers to MF30 increased. After 14 d suspension the immunoreactivity of the bag 1 fiber and bag 2 fiber to ALD58 decreased remarkably, even disappeared. While all of the intrafusal fibers reacted to MF30 and the immunoreactivity increased obviously. Conclusion The expression level and patterns of MHC in intrafusal fibers of rat soleus muscle changed after simulated weightlessness.

关 键 词：失重模拟 尾吊 肌球蛋白 重链表达 ALD58 MF30 肌梭 

分 类 号：R852.22[医药卫生—航空、航天与航海医学] R852.4[医药卫生—临床医学]