强骨抗萎方对模拟失重大鼠骨形态学的影响  

Effects of Qiang Gu Kang Wei Prescription on Morphology of Bone in Rats under Simulated Weightlessness

作　　者：李勇枝[1] 石宏志[1] 范全春[1] 陶英[1] 白桂娥[1] 段渊儒[1] 谢俊水[1] 

机构地区：[1]航天医学工程研究所,北京100094

出　　处：《航天医学与医学工程》2003年第4期272-276,共5页Space Medicine & Medical Engineering

基　　金：中国载人航天工程基金资助

摘　　要：目的研究中药复方强骨抗萎方对尾吊大鼠骨形态学的影响。方法用大鼠尾吊 - 30° 2 1d模拟失重 ,观察强骨抗萎方对大鼠胫骨干的骺端骺板、骺板下 3mm处骨皮质及骨膜厚度的影响 ,并进行胫骨组织学和超微结构的观察。结果与正常对照组相比 ,尾吊大鼠胫骨干的骺端骺板和骺板下 3mm处的骨皮质均变薄 (P <0 .0 1或P <0 .0 5 )。组织学观察显示 ,骨皮质内未钙化的基质多 ,包埋在基质中的骨细胞数量少 ,且较幼稚 ;成骨细胞少且小 ;破骨细胞多而大 ;横截面哈佛系统不规则。超微结构提示成骨细胞功能低下 ,而破骨细胞功能活跃。与模型组比较 ,中药复方大、中、小各剂量组胫骨干的骺端骺板和骺板下 3mm处的骨皮质厚度均有不同程度的增加 (P <0 .0 1或P <0 .0 5 ) ;光镜及电镜观察结果表明其形态也比较接近正常。结论该方可改善大鼠因尾吊引起的骨形态学的异常改变 。Objective To investigate effects of Qiang Gu Kang Wei prescription on bone morphology in tail suspended rats. Method Rats were tail suspended (-30°) for 21d to simulate weightlessness. The effects of this prescription on thickness of periost and cortex at epiphyseal plate of tibial shaft and 3 mm under it were examined.The effects of this prescription on histology and ultrastructure of tibia were also observed. Result Compared with the normal control, cortex at both epiphyseal plate of tibial shaft and 3 mm under it in suspended rats thinned significantly(P<0.01 or P<0.05).Histological observation showed that in bone of suspended rats, uncalcific matrix was more, bone cells′ number was lower and their appearance was more infantile, osteoblasts were less and smaller, while osteoclasts were more and larger, haversian system of transection also showed more irregular than in bone of normal control. Ultrastructure observation showed that the function of osteoblasts was inhabited and that of osteoclasts was active. All the three doses groups of the prescription could increase cortex at either epiphyseal plate of tibial shaft or 3 mm under it (P<0.01 or P<0.05) compared with the tail suspended group. The appearance of bone in the three groups was improved as well under optical or electron microscope. Conclusion This prescription could improve abnormal change in bone morphology resulted from being tail suspended in rats and make it normal.

关 键 词：失重模拟 强骨抗萎方 尾吊 骨组织 形态学 成骨细胞 破骨细胞 

分 类 号：R856.2[医药卫生—航空、航天与航海医学] R854[医药卫生—临床医学]