川芎嗪对大鼠心肌缺血损伤的拮抗作用  

Protective effects of ligustrazine against myocardial ischemia injury of rat

作　　者：黎玉[1] 万福生[1] 万义福[2] 

机构地区：[1]江西医学院,江西南昌330006 [2]江西省肺科医院,江西南昌330006

出　　处：《中成药》2003年第8期646-648,共3页Chinese Traditional Patent Medicine

基　　金：江西省自然科学基金资助项目 (0 0 40 0 5 4)

摘　　要：目的 :研究川芎嗪对异丙肾上腺素致大鼠心肌缺血损伤的保护作用。方法 :采用皮下注射异丙肾上腺素 (ISP ,5mg·kg-1) ,2 4h后再注射同剂量一次 ,造成心肌缺血损伤模型。观测心肌线粒体中Ca2 + ATP酶、Ca2 + Mg2 + ATP酶活力以及Ca2 + 含量的变化。采用免疫组化法检测Bcl 2和Bax蛋白水平的变化。结果 :川芎嗪保护组心肌线粒体Ca2 + ATP酶、Ca2 + Mg2 + ATP酶活力较缺血组均有显著性升高 (P <0 .0 1) ,川芎嗪可稳定心肌线粒体Ca2 + 含量 ,增加缺血心肌组织中Bcl 2的表达 (P <0 .0 1) ,对Bax的表达影响不大 (P >0 .0 5 )。结论 :川芎嗪对大鼠心肌缺血损伤具有保护作用 ,该作用可能与提高心肌线粒体Ca2 + ATP酶、Ca2 + Mg2 + ATP酶活力及调控Bcl 2基因的表达有关。Objective: To observe the protective effect of ligustrazine on myocardial ischemia rat induced by isoproterenol. Methods : The model of myocardial ischemia was induced by subtcuaneous injection of isoproterenol (ISP, 5mg·kg -1 ) into rats and repeated the same dose 24 hours later. The activities of Ca 2+ -ATPase、Ca 2+ -Mg 2+ -ATPase and the contents of Ca 2+ in the myocardial mitochondria were observed, respectively. The changes of protein expression of bcl-2 and bax genes were detected by immunohistochemical staining. Results : In the ligustrazine treated group, as compared with those of the model, the activities of Ca 2+ -ATPase、Ca 2+ -Mg 2+ -ATPase in the myocardial mitochondria increased significantly( P <0.01). Ligustrazine could stabilize the content of Ca 2+ in the myocardial mitochondria, enhance the protein expression of bcl-2( P <0.01), while it had no significance with bax expression ( P >0.05). Conclusion : Ligustrazine possesses protective effects against myocardial ischemia injury via increasing the activities of Ca 2+ -ATPase、Ca 2+ -Mg 2+ -ATPase in the myocardial mitochondria and influencing the expression of bcl-2.

关 键 词：川芎嗪 心肌缺血 线粒体 腺苷三磷酸酶类 基因表达 大鼠 

分 类 号：R542.2[医药卫生—心血管疾病] R965[医药卫生—内科学]