家蚕对马尾松毛虫质型多角体病毒的敏感性  

The sensitivity of Bombyx mori larvae to Dendrolimus punctatus cytoplasmic polyhedrosis virus

作　　者：洪靖君[1,2] 段家龙[1] 赵淑玲[3] 彭辉银[3] 

机构地区：[1]安徽农业大学蚕业丝绸系 [2]中国科学院武汉病毒研究所生物防治室,武汉430071 [3]中国科学院武汉病毒研究所生物防治室

出　　处：《昆虫学报》2003年第4期409-416,共8页Acta Entomologica Sinica

基　　金：科学技术部重点项目 (K2 0 0 0 0 3 3 5 );云南省院合作项目 ( 2 0 0YKS0 2 )

摘　　要：用虫体克隆技术 ,对马尾松毛虫质型多角体病毒湖南株 (DpCPV HN)进行了分离纯化 ,鉴定为质型多角体病毒 1型。以家蚕春蕾×镇珠杂种F1 代及自交的F2 代 4或 5日龄幼虫进行毒力测定 ,以纯化的家蚕质型多角体病毒对F1 代幼虫的毒力测定为对照。结果表明 :家蚕品种春蕾×镇珠对家蚕质型多角体病毒敏感 ,马尾松毛虫质型多角体病毒湖南株能引起其感染发病 ;马尾松毛虫质型多角体病毒湖南株感染家蚕品种春蕾×镇珠F1 代幼虫和F2 代幼虫 2 8天后的半致死剂量(LD50 )分别为 885个和 18个CPB (质多角体 ) ,前者为后者的 4 9倍。马尾松毛虫质型多角体病毒湖南株感染后的家蚕 ,其结茧率、化蛹率、羽化率、全茧量、茧层量和单蛾产卵数均有所下降 ,全茧量、茧层量、茧层率和单蛾产卵数与病毒感染剂量之间无显著关联。Dendrolimus punctatus cytoplasmic polyhedrosis virus strain Hunan (DpCPV HN) was cloned in vivo and identified as a type 1 cypovirus. Fourth\| or 5th day post hatching F 1 and F 2 silkworms from an generation F 1 that had previously been infected by DpCPV HN, and two other type 1 cypoviruses from Bombyx mori (BmCPV 1) and D. punctatus (DpCPV HN) were selected for the infection test. The results indicate that the breed of B. mori tested in this study was very sensitive to BmCPV 1, and could also be infected by DpCPV HN. Based on the accumulative total mortalities of F 1 and F 2 generations of B. mori infected by DpCPV HN over 28 days post infection,the 50% lethal doses (LD 50 ) of DpCPV HN of F 1 and F 2 generations were 885 and 18 CPBs respectively. Obviously, the F 2 silkworm larvae from DpCPV infected generation F 1 were more sensitive to DpCPV HN than those of the F 1 generation. However, the sensitivity of the F 2 generation to DpCPV HN was still inferior to that of the F 1 to BmCPV 1. To some degree, the ratio of cocoons,ratio of pupae,ratio of moths to pupae, cocoon weight, cocoon shell weight, ratio of cocoon shell weight, and number of eggs from single female moths of the F 1 and F 2 generations were affected after infection by DpCPV HN. The correlations between cocoon weight, cocoon shell weight, ratio of cocoon shell weight,number of eggs from single female moths of silkworms and inoculation doses of DpCPV HN were not obvious.

关 键 词：马尾松毛虫质型多角体病毒 家蚕质型多角体病毒 家蚕 敏感性 虫体克隆 

分 类 号：Q965.9[生物学—昆虫学]