光增白剂对甜菜夜蛾围食膜结构的作用与影响  

Effects of fluorescent brightener on the peritrophic membrane structure of Spodoptera exigua

作　　者：朱蓉[1] 彭建新[1] 洪华珠[1] 

机构地区：[1]华中师范大学昆虫学研究所,武汉430079

出　　处：《昆虫学报》2003年第4期424-428,共5页Acta Entomologica Sinica

基　　金：湖北省自然科学基金 ( 2 0 0 0J112 );教育部科学技术重点项目 ( 0 2 192 )

摘　　要：应用环境扫描电镜和生化技术研究了甜菜夜蛾Spodopteraexigua正常围食膜和光增白剂M2R处理围食膜的形态、结构和组成。结果表明 :正常的围食膜表面光滑致密、无孔洞和缝隙 ;光增白剂处理的围食膜产生了孔缝。正常围食膜所含蛋白质的种类很多 ,经SDS PAGE测定至少有 17条多肽 ,分子量多在 97 4kD以下 ,围食膜蛋白质的含量约为 4 1 98% ,糖的含量约为 2 0 5 %。光增白剂可以解离大部分围食膜蛋白 ,液滴法喂食幼虫蓝色葡聚糖 2 0 0 0进一步证实了光增白剂能破坏围食膜的完整性。Environmental scanning electron microscopy and biochemical techniques were employed to analyze the structure of the normal and fluorescent brightener 28(M2R) treated peritrophic membrane(PM) of Spodoptera exigua. The normal PM surface was smooth and wrinkled without pores or slits while the treated PM surface had pores and slits.There were many types of protein in the PM.SDS\|PAGE separation of the PM suggest that there are at least 17 polypetides,most of them less than 97 4?kD.Proteins formed 41 98% of the total mass of the PM and carbohydrates 2 05%.The PM proteins could be dissociated from the PM in vitro by M2R.Droplet feeding fifth instar larvae with M2R greatly increased the permeability of the PM.

关 键 词：甜菜夜蛾 围食膜 光增白剂 环境扫描电镜 

分 类 号：Q962[生物学—昆虫学]