反相高效液相测定洛伐他汀方法的改良  

Improvement of RT-HPLC on determination of lovastatin

作　　者：谢虹[1] 邱相君[2] 付琳[3] 王桂珍[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生教研室,武汉430030 [2]温州医学院药理学教研室 [3]华中科技大学同济医学院临床药理教研室

出　　处：《中国公共卫生》2003年第7期814-815,共2页Chinese Journal of Public Health

摘　　要：目的　探索采用反相高效液相色谱法 (HPLC) ,提高检出血浆洛伐他汀浓度及缩短测定时间。方法　HPLC条件 :色谱柱YWG -C1 8(1 0 μm) ;流动相∶甲醇∶水 (83∶1 7,V/V) ;柱温 2 5℃ ;检测波长 2 37nm。血浆经环己烷和异丙醇萃取浓缩后 ,直接进样测定洛伐他汀的浓度。结果　血浆洛伐他汀浓度在 2 0～ 80 0 μg/L范围内线性关系良好 ,回归方程式 ^y =2 4 1 56 +1 97 2 5x(r=0 9991 ) ;最低检测限为 2 0 μg/L ;平均回收率为 (96 0 1± 7 1 7) %。测定一个样品的时间只需 6min。结论　血浆前处理充分 ,流动相甲醇与水比例适当是提高检测限和缩短测定样品时间的关键 。Objective To improve the detection concentration and time of plasma lovastatin by studying the RP-HPLC method.Methods The condition of HPLC:The analytical column was packed with YWG-C18(10?μm).The mobile phase was methanol-water(83∶17,V/V).The temperature of column was 25?℃.The compounds were quantified using a ultraviolet detector operated at 237?nm?The lovastatin in plasma sample extracted by cycllohexane-isopropyl alcohol.Results The liner range of plasma lovastatin was 2.0-80?ng/ml.The regression recation was =241 56+197 25x( r =0 999?1).The limit of detection was 2 0?μg/L and the mean recovery was (96 01±7 17)%.The determination of a sample only needed 6?min.Conclusion Controlling the pretreatment of plasma and the ratio of methanol to water was the way to enhance the detection limit and shorten detection time.

关 键 词：洛伐他汀 HPLC 方法改良 血浆 

分 类 号：R917[医药卫生—药物分析学]