不同地区福氏2a志贺菌质粒DNA多态性分析  

Analysis on plasmid DNA polymorphism of S.flexneri 2a from different areas

作　　者：郝加虎[1] 叶冬青[1] 王红[2] 钟文龙[3] 夏桂枝[2] 黄芬[1] 王玉琴[1] 俞守义[2] 

机构地区：[1]安徽医科大学公共卫生学院流行病与卫生统计学系,合肥230032 [2]第一军医大学流行病学教研室 [3]广东省江门市卫生防疫站

出　　处：《中国公共卫生》2003年第7期809-811,共3页Chinese Journal of Public Health

摘　　要：目的　对福氏 2a志贺菌质粒DNA进行基因分型 ,提高福氏 2a志贺菌痢疾暴发流行时同源克隆的鉴定分析水平。方法　对 93株分离自不同地区、不同时间的福氏 2a志贺菌用质粒图谱和限制性内切酶图谱法进行基因分型和同源克隆鉴定。结果　质粒DNA分析表明 ,不同地区、不同时间分离的 93株福氏 2a志贺菌具有 1 3种不同的质粒DNA图谱特征 (PLT1～PLT1 3型 )和 1 5种限制性内切酶图谱特征 (ReT1～ReT1 5型 )。结论　 93株来自不同地区、分离自不同时间的福氏 2a志贺菌分属于 1 5个克隆群 ,PLT型和ReT型分布呈现多态性特征 ,PLT1 /ReT1型是海南、江门、蚌埠、合肥、安庆地区福氏 2a志贺菌的优势克隆群组。表型相同的福氏 2a志贺菌实际上是遗传结构多态性群体的集合 。Objective To identify homogenous clones of S flexneri 2a,using gene distribution polymorphism of plasmid DNA of S.flexneri 2a.Methods 93 strains of S.flexneri 2a isolated from different areas at different time were detected by plasmid profiles and restriction endonuclease profiles to identify genetic type and homogenous clones of S.flexneri 2a.Results Plasmid DNA analysis showed that the 93 strains of S.flexneri 2a isolated from different areas at different time possessed 13 different plasmid profile features (PLT1/-PLT13 type) and 15 different restriction endonuclease profile features (ReT1-ReT15 type).Conclusion The 93 strains of S.flexneri 2a isolates obtained from different areas and at different time fell into 15 subclonial clusters.Although these isolates shared a common predominant clonial cluster(PLT1/ReT1 type)in Hainan,Jiangmen,Bengbu,Hefei,Anqing.The same phenotyping S.flexneri 2a was actually composed of various genetic polymorphism construction clonial clusters,there existed obvious difference in the clonial distribution and the population genetic construction.Phenotyping analysis for the determination of source of infection and the route of transmission will result in a one-side view.

关 键 词：福氏2A志贺菌 质粒 基因分型 

分 类 号：R378.25[医药卫生—病原生物学]