SPR生物传感器测定胰蛋白酶与抑制剂的相互作用  

Study of the interaction between trypsin and inhibitors by using SPR based biosensor

作　　者：杨帆[1] 杨秀荣[1] 

机构地区：[1]中国科学院长春应用化学研究所电分析化学国家重点实验室,长春130022

出　　处：《化学传感器》2003年第2期32-36,共5页Chemical Sensors

基　　金：国家自然科学基金(批准号:20075027);国家重点基础研究规划项目(编号:2001CB5102)资助课题

摘　　要：该文报道了利用基于表面等离子体共振(SPR)原理的生物传感器BIAcore测定胰蛋白酶与两种胰蛋白酶抑制剂之间相互作用的动力学性质。测定结果表明,抑制剂与酶之间的亲和常数与抑制剂对酶的抑制作用大小具有相关性。该方法测定具有快速、简便的特点。In this paper, we studied the kinetic characterization of interaction between trypsin and its two inhibitors by using surface plasmon resonance - based biosensor BIAcore. The results indicated that there is correlation between affinity constants of the interactions of trypsin and its two inhibitors and inhibition ability of inhibitors. The measurement can be carried out easily and quickly.

关 键 词：SPR生物传感器 测定 胰蛋白酶 抑制剂 表面等离子体共振 

分 类 号：R657.51[医药卫生—外科学]