过氧化氢对豚鼠耳蜗功能及形态的影响  

Effects of Hydrogen Peroxide on Function and Morphology of Guinea Pig Cochlea

作　　者：赖丹[1] 黎万荣[1] 李兴启[2] 

机构地区：[1]四川省泸州医学院附属医院耳鼻咽喉-头颈外科,泸州646000 [2]解放军总医院耳鼻咽喉科研究所

出　　处：《听力学及言语疾病杂志》2003年第3期189-192,共4页Journal of Audiology and Speech Pathology

基　　金：国家自然科学基金资助项目 (批准号 3 0 0 70 812 )

摘　　要：目的　在体观察过氧化氢 (H2 O2 )对豚鼠耳蜗功能及形态的影响。方法　实验动物分为 4组 ,分别灌流人工外淋巴液 (artificialperilymph ,APL) ,5 0 μMH2 O2 ,10 0 μMH2 O2 和 2 0 0 μMH2 O2 (H2 O2 均溶于APL中 ) ,并用PI和HO33342双染色方法观察H2 O2 引起的内耳细胞损伤情况。结果　所有H2 O2 灌流组复合动作电位 (CAP)阈移和耳蜗微音电位 (CM)幅度变化与人工外淋巴液组比较差异有显著性 ,且各H2 O2 组的变化呈现出浓度依赖性 ;PI和HO33342双染色方法发现外毛细胞是H2 O2 攻击的主要靶细胞 ,而Hensen细胞未见任何损伤痕迹。结论　H2 O2 可导致耳蜗功能下降及耳蜗毛细胞损伤 ;Objective To investigate the effects of hydrogen peroxide on function and morphology of guinea pig cochlea in vivo.Methods Animals were divided into four groups and their cochleas were perfused with artificial perilymph(AP), 50 μM H 2O 2, 100 μM H 2O 2 and 200 μM H 2O 2 resolved in AP separately. The compound action potential(AP) and cochlear microphonic(CM) were recorded after two-hours' perfusion. Then hydrogen peroxide-induced cell damage in the inner ear was investigated with morphologic method.Results In all H 2O 2 groups, CAP threshold shifts and CM amplitude shifts were significantly greater than that of APL group. The effect of H 2O 2 perfusion on cochlear function showed dose-dependence. OHCs were major targets of H 2O 2-induced cell death, while Hensen's cells did not show any signs of damage in the presence of H 2O 2. Conclusion Hydrogen peroxide, as an ubiquitous reactive oxygen species(ROS), can induce cochlea dysfunction and damage of cells in guinea pig inner ear. Hensen's cells maybe more resistant to the damage of ROS than hair cells.

关 键 词：过氧化氢 豚鼠 耳蜗功能 耳蜗形态 影响 耳蜗微音电位 复合动作电位 

分 类 号：R764.35[医药卫生—耳鼻咽喉科]