卫氏并殖吸虫成虫的特异性诊断抗原  

The specific diagnostic antigens of Paragonimus westermani adult worms

作　　者：张灯[1] 王中全[1] 崔晶[1] 范清堂[2] 毛福荣[1] 晋雪香[1] 

机构地区：[1]郑州大学基础医学院寄生虫学教研室,郑州450052 [2]河南省分子医学重点学科开放实验室,郑州450052

出　　处：《郑州大学学报（医学版）》2003年第4期538-540,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技攻关计划项目 991170 411

摘　　要：目的 :寻找诊断并殖吸虫病的特异性抗原。方法 :应用SDS PAGE和Westernblot对卫氏并殖吸虫成虫可溶性抗原中的蛋白组分进行分析。结果 :卫氏并殖吸虫成虫可溶性抗原蛋白经SDS PAGE后显示 14条蛋白带 ,其中相对分子质量为 5 30 0 0、310 0 0、2 5 0 0 0、16 0 0 0、110 0 0是主带 ;相对分子质量为 5 30 0 0和 340 0 0的条带可与华支睾吸虫病患者血清反应 ;10 80 0 0、94 0 0 0、6 5 0 0 0的条带可与血吸虫病患者血清反应 ;5 80 0 0、5 30 0 0、4 30 0 0的条带可与旋毛虫感染的大鼠血清、小鼠血清及旋毛虫病患者血清反应。 370 0 0的蛋白带只能被斯氏狸殖吸虫感染的大鼠血清和并殖吸虫病患者血清所识别 ,而不与华支睾吸虫病、血吸虫病患者血清、旋毛虫感染的大鼠和小鼠血清、旋毛虫病患者血清、正常大鼠、小鼠血清及正常人血清发生交叉反应。结论 :卫氏并殖吸虫成虫可溶性抗原中相对分子质量为 370 0 0的蛋白组分为卫氏并殖吸虫成虫的特异性抗原 。Aim: To find out the specific antigens for immunodiagnosis of paragonimiasis.Methods: The soluble antigens of Paragonimus westermani adult worms were analyzsed by SDS PAGE and immunoblotting.Results: SDS PAGE revealed that the soluble antigens of P. westermani adult worms had 14 protein bands, among which the protein bands with molecular weight (MW) of 53 000,31 000,25 000,16 000,and 11 000 were the major bands. The immunoblotting results showed that the protein bands with MW of 53 000 and 34 000 in P. westermani adult worm soluble antigens cross reacted with sera from patients with clonorchiasis. The protein bands with MW of 108 000,94 000 and 65 000 cross reacted with sera from patients with schistosomiasis. All of the protein bands with MW of 58 000,53 000,and 43 000 cross reacted with sera from the rats and mice infected with T. spiralis and patients with trichinellosis. The protein component with MW of 37 000 was only reacted with sera from the rats infected with P. skrjabini and patients with paragonimiasis, but not reacted with sera from patients wth clonorchiasis, schistosomiasis, trichinellosis,sera from rats and mice infected with T. spiralis ,and sera from normal rats, mice, and persons.Conclusions:The protein component with MW of 37 000 in P. westermani adult worm soluble antigens is the specific antigen for P. westermani adult worm.It could be applied to the immunodiagnosis and seroepidemiological investigation of paragonimiasis.

关 键 词：卫氏并殖吸虫成虫 特异性诊断抗原 并殖吸虫病 成虫抗原 免疫印迹 

分 类 号：R532.22[医药卫生—内科学]