Cytomorphology and Cytochemical Localization of K-type and T-type Cytoplasmic Male Sterile Pollens in Wheat  

作　　者：YAO Ya-qin, ZHANG Gai-sheng, LIU Hong-wei and WANG Jun-wei(Northwest Sci-Tech University of Agriculture and Forestry , Yangling 712100 , P. R. China) 

出　　处：《Agricultural Sciences in China》2003年第6期591-596,T001-T003,共9页中国农业科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(39770366);Research Program of Agricultural Biotechnological Breeding Center in Yangling,China.

摘　　要：By using the technology of microscopy, electron microscopy and enzyme cytochemical localization , comparison was made of the cytomorphology, ATPase and cytochrome oxidase activity in pollens during microspore formation and pollen development, for K-type cytoplasmic male sterile(CMS)lines,T-type CMS lines and their maintainer lines on wheat. The results indicated that pollen abortion of the T-type CMS lines mainly occurred at the later mononucleate pollen stage, and cytomorphological changes of the abortive pollen began with a vacuolar membrane, and pollen aborting was related to a lack of ATPase activity in the nucleus and nucleolus during the mononucleate pollen stage. Pollen abortion of the K-type CMS lines took place mainly during the later binucleate and trinucleate stages, and cytomorphological changes of the abortion first began with mitochondria. The abortion was related to intine disruption.By using the technology of microscopy, electron microscopy and enzyme cytochemical localization , comparison was made of the cytomorphology, ATPase and cytochrome oxidase activity in pollens during microspore formation and pollen development, for K-type cytoplasmic male sterile(CMS)lines,T-type CMS lines and their maintainer lines on wheat. The results indicated that pollen abortion of the T-type CMS lines mainly occurred at the later mononucleate pollen stage, and cytomorphological changes of the abortive pollen began with a vacuolar membrane, and pollen aborting was related to a lack of ATPase activity in the nucleus and nucleolus during the mononucleate pollen stage. Pollen abortion of the K-type CMS lines took place mainly during the later binucleate and trinucleate stages, and cytomorphological changes of the abortion first began with mitochondria. The abortion was related to intine disruption.

关 键 词：Wheat pollen CMS CYTOMORPHOLOGY CYTOCHEMISTRY 

分 类 号：S512.1[农业科学—作物学]