Copper-Controllable, Site-Specific DMA Excision in Transgenic Plants  

作　　者：PENG Xiang-lei, LIANG Bin, CHEN Ming, HU Yuan-lei and LIN Zhong-ping(National Key Laboratory of Protein Engineering and Plant Genetic Engineering , Peking University , Beijing 100871 , P. R. China) 

出　　处：《Agricultural Sciences in China》2003年第6期597-601,共5页中国农业科学（英文版）

基　　金：supported by the 863 Program,China(2002AA224011 and 2001AA212161).

摘　　要：A copper-inducible, Cre-loxP recombination-mediated DNA excision system has been developed in transgenic tobacco plants. The copper inducible system derived from yeast was used for the control of the expression of the Cre recombinase. Upon copper induction, the GVS reporter gene expression unit flanked by two direct lox sites was excised from the transgenic tobacco genome. Quantitative fluorometric GUS assays, Northern blot and PCR analyses showed a high-efficient, copper-dependent and Cre-loxP mediated DNA recombination in all the tested transgenic lines. The copper inducible foreign gene excision might be of great potential in genetic control of transgenic crops.A copper-inducible, Cre-loxP recombination-mediated DNA excision system has been developed in transgenic tobacco plants. The copper inducible system derived from yeast was used for the control of the expression of the Cre recombinase. Upon copper induction, the GVS reporter gene expression unit flanked by two direct lox sites was excised from the transgenic tobacco genome. Quantitative fluorometric GUS assays, Northern blot and PCR analyses showed a high-efficient, copper-dependent and Cre-loxP mediated DNA recombination in all the tested transgenic lines. The copper inducible foreign gene excision might be of great potential in genetic control of transgenic crops.

关 键 词：Transgenic plants Copper-inducible system MRE CRE-LOXP DNA recombination 

分 类 号：Q943.2[生物学—植物学]