Microbial Transformation of Acetophenone to 2-Phenylethanol  

作　　者：蔺兆星 郝宁 陈国强 

机构地区：[1]Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China

出　　处：《Tsinghua Science and Technology》2003年第4期471-475,共5页清华大学学报（自然科学版（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.2 0 0 74 0 2 0 )

摘　　要：Microorganisms are commonly used to transform chemicals to chiral compounds. Pseudomonas cepacia 1813, Pseudomonas stutzeri 1317, Saccharomyces cerevisiae 1912, and 5 Escherichia coli strains were used to transform acetophenone to 2 phenylethanol. The results show that the E. coli strains have the poorest biotransformation ability. P. stutzeri 1317 provides the best transformation ability with a transformation rate of 30% achieved with either whole or broken P. stutzeri 1317 cells for a reaction pH of 8.2 and an initial acetophenone concentration of 2 g/L. An acetophenone concentration of 10 g/L strongly inhibits the biotransformation for a pH of 8.2. Crude alcohol dehydrogenase obtained from S. cerevisiae 1912 transforms acetophenone to 2 phenylethanol when nicotinamide adenine dinucleotide reduced (NADH) is added.Microorganisms are commonly used to transform chemicals to chiral compounds. Pseudomonas cepacia 1813, Pseudomonas stutzeri 1317, Saccharomyces cerevisiae 1912, and 5 Escherichia coli strains were used to transform acetophenone to 2 phenylethanol. The results show that the E. coli strains have the poorest biotransformation ability. P. stutzeri 1317 provides the best transformation ability with a transformation rate of 30% achieved with either whole or broken P. stutzeri 1317 cells for a reaction pH of 8.2 and an initial acetophenone concentration of 2 g/L. An acetophenone concentration of 10 g/L strongly inhibits the biotransformation for a pH of 8.2. Crude alcohol dehydrogenase obtained from S. cerevisiae 1912 transforms acetophenone to 2 phenylethanol when nicotinamide adenine dinucleotide reduced (NADH) is added.

关 键 词：ACETOPHENONE 2  phenylethanol BIOTRANSFORMATION Pseudomonas stuzteri 

分 类 号：O625[理学—有机化学]