Characterization of Adherent Nonhematopoietic Cells Derived from Human Umbilical Cord Blood  

作　　者：安小惠 蔡国平 

机构地区：[1]Department of Biological Sciences and Technology, Tsinghua University, Beijing 100084, China

出　　处：《Tsinghua Science and Technology》2003年第4期412-416,共5页清华大学学报（自然科学版（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.39970 70 3)

摘　　要：To confirm and characterize the adherent fibroblast like progenitors in human umbilical cord blood, we isolated mononuclear cells from human umbilical cord blood by Ficoll Hypaque. Two main morphologically different kinds of cells were formed by culturing the cells in collagen coated 24 well plastic dishes and flasks. One type was the adherent fibroblast like cells, while the other was loosely adherent clonally expanded round cells. Our experiments demonstrate that the adherent fibroblast like cells possess multilineage potential, including the ability to differentiate into endothelial like cells and to express the mesenchymal cell marker.To confirm and characterize the adherent fibroblast like progenitors in human umbilical cord blood, we isolated mononuclear cells from human umbilical cord blood by Ficoll Hypaque. Two main morphologically different kinds of cells were formed by culturing the cells in collagen coated 24 well plastic dishes and flasks. One type was the adherent fibroblast like cells, while the other was loosely adherent clonally expanded round cells. Our experiments demonstrate that the adherent fibroblast like cells possess multilineage potential, including the ability to differentiate into endothelial like cells and to express the mesenchymal cell marker.

关 键 词：human umbilical cord blood mesenchymal cells endothelial cells 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]