二氧化硫对小鼠脑细胞DNA的损伤作用  

Damage Effects of Sulfur Dioxide Inhalation on DNA of Brain Cells in Mice

作　　者：张波[1] 刘承芸[1] 孟紫强[1] 

机构地区：[1]山西大学环境医学与毒理学研究所,山西太原030006

出　　处：《山西大学学报（自然科学版）》2003年第3期257-259,共3页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(30070647);山西省自然科学基金

摘　　要：运用单细胞凝胶电泳技术(又称彗星试验)研究了SO2吸入对小鼠脑细胞DNA的损伤.研究结果表明,SO2可引起小鼠脑细胞彗星尾长显著大于对照组,且随SO2吸入浓度的增加,脑细胞彗星尾长度也增加,具有剂量效应关系.结果还表明,SO2对雄性小鼠脑细胞DNA损伤比雌性小鼠严重,存在性别差异.Damage effect of SO2 at different conentration(28 mg/m3,56 mg/m3,112 mg/m3 and 168 mg/m3)inhalation on DNA of brain cells from mice were studied with the single cell microgel electrophoresis technique(SCGE, comet assay).The results showed lengths of comet tails were increased obviously after SO2 inhalation,and the lengths of comet tail increased correspondingly according to SO2 concentrations increased.At lower SO2 levels,degree I DNA strand breaks were caused mainly,but as SO2 concentration increased,the proportion of cells with degree Ⅱ and drgreeⅢ DNA strand breaks increased.Some sex defferences in DNA damage were also found.DNA damage of male mice in brain was more serious than that of female mice.

关 键 词：二氧化硫 小鼠 脑细胞 DNA损伤 单细胞凝胶电泳 剂量效应关系 大气污染物 损伤作用 毒性 

分 类 号：X511.032.2[环境科学与工程—环境工程]