实验性早期心肌梗死的免疫组化研究  

Immunohistochemical study for the postmortem diagnosis of early myocardial infarction

作　　者：申成斌 王兆福 胡丙杰[3] 

机构地区：[1]河南省公安刑事科学技术研究所,河南郑州450003 [2]河南省直属机关第二医院,河南郑州450003 [3]广东省广州市卫生局,广东广州510080

出　　处：《河南医学研究》2003年第2期119-123,共5页Henan Medical Research

摘　　要：目的 :对纤维连接蛋白 (Fn)、纤维蛋白原 (Fg)、补体 (C5)、肌红蛋白 (Mb)、肌动蛋白 (HHF3 5 )、结蛋白(Dm)六种指标在诊断早期心肌梗死的敏感性进行研究。方法 :建立大鼠急性心肌缺血模型 ,应用免型组织化学方法和图像分析与统计学处理系统 ,检测缺血心肌细胞内Mb、HHF3 5、Dm的缺失面积和Fn、Fg、C5的阳性反应面积。结果 :Mb、HHF3 5、Dm在大鼠急性心肌缺血 15min即可出现缺失 ,Fg、C5在心肌缺血 15min即可出现阳性反应 ,而Fn直到心肌缺血 3h才出现阳性反应。随缺血时间延长 ,Dm、HHF3 5、Mb的缺失面积和Fg、C5、Fn的阳性反应面积逐步增大。结论 :Dm、HHF3 5、Mb、Fg、C5是反映早期心肌梗死的灵敏的形态指标 。Objective: To investigate the sensitivity of fibronection(Fn),fibrinogen(Fg),complement(C 5),Myoglobin (Mb),Actin (HHF35) and desmin (Dm) for the postmortem diagnosis of early myocardial infarction.Methods: The myocardial ischemia model was established in rats,and the depletion areas of Mb HHF35 and Dm,the positive reaction areas of Fn,Fg and C 5 in the ischemic myocardial cells were studied with immunohistochemistry,image analysis technique and statistical system.Results: The depletion of Dm,HHF35 and Mb,and the positive staining of Fg and C 5 in ischemic myocardial cells were found at 15 min after the myocardial ischemia,but the positive saining of Fn occurred till 3 hours after myocardial ischemia.With the time prolonging,the areas of the depletion of Dm,HHF35,Mb and the positive staining of Fg, C 5 and Fn gradually enlarged.Conclusion: It is suggested that Mb,HHF35,Dm,Fg and C 5 are more sensitive markers than Fn for postmortem diagnosis of myocardial infarction.

关 键 词：实验 早期心肌梗死 免疫组织化学 纤维连接蛋白 纤维蛋白原 补体 肌红蛋白 

分 类 号：R542.22[医药卫生—心血管疾病]