Establishment of transgenic mice carrying the gene of human nuclear receptor NR5A2 (hB1F)  

作　　者：Shui-LiangWang HuaYang You-HuaXie YuanWang Jian-ZhongLi LongWang Zhu-GangWang Ji-LiangFu 

机构地区：[1]DepartmentofMedicalGenetics,SecondMilitaryMedicalUniversity,Shanghai200433,China [2]InstituteofBiochemistryandCellBiology,ShanghaiInstituteforBiologicalSciences,ChineseAcademyofSciences,Shanghai200031,China [3]ShanghaiNanfangResearchCenterforModelOrganisms,Shanghai201203,China

出　　处：《World Journal of Gastroenterology》2003年第6期1333-1336,共4页世界胃肠病学杂志（英文版）

基　　金：the National Natural Science Foundation of China,No.39830360;the National "863"High Technology Research and Development Program of China,No.2001AA221261

摘　　要：AIM: Human hepatitis B virus enhancer II B1 binding factor (hBIF) was cloned and characterized as a novel member of the Ftz-F1 (NR5A) nuclear receptor subfamily. Although progresses have recently been made, its biological function remains largely unidentified. The aim of this study was to establish an hBIF transgenic mouse model to promote the functional study of hB1F.METHODS: Transgene fragments were microinjected into fertilized eggs of mice. The manipulated embryos were transferred into the oviducts of pseudopregnant female mice.The offsprings were identified by PCR and Southern blot analysis. Transgene expression was analyzed with RT-PCR and Western blot analysis. Transgenic founder mice were used to establish transgenic mouse lineages. The F1 and F2 mice were identified by PCR analysis.RESULTS: Seven mice were identified as carrying copies of transgene. RT-PCR and Western blotting results showed that the transgene was expressed in heart, liver, lung, kidney and stomach in one of the transgenic mouse lineages.Genetic analysis of the transgenic mice demonstrated that the transgene was integrated into the chromosome at a single site, and was transmitted stably.CONCLUSION: In this study we established an hB1F transgenic mouse model, which will facilitate the investigation of the biological function of hB1F in vivo.AIM:Human hepatitis B virus enhancer Ⅱ B1 binding factor (hBIF) was cloned and characterized as a novel member of the Ftz-F1 (NR5A) nuclear receptor subfamily.Although progresses have recently been made,its biological function remains largely unidentified.The aim of this study was to establish an hBIF transgenic mouse model to promote the functional study of hBIF. METHODS:Transgene fragments were microinjected into fertilized eggs of mice.The manipulated embryos were transferred into the oviducts of pseudopregnant female mice. The offsprings were identified by PCR and Southern blot analysis.Transgene expression was analyzed with RT-PCR and Western blot analysis.Transgenic founder mice were used to establish transgenic mouse lineages.The F1 and F2 mice were identified by PCR analysis. RESULTS:Seven mice were identified as carrying copies of transgene.RT-PCR and Western blotting results showed that the transgene was expressed in heart,liver,lung,kidney and stomach in one of the transgenic mouse lineages. Genetic analysis of the transgenic mice demonstrated that the transgene was integrated into the chromosome at a single site,and was transmitted stably. CONCLUSION:In this study we established an hBIF transgenic mouse model,which will facilitate the investigation of the biological function of hBIF in vivo.

关 键 词：乙型肝炎病毒 hBIF NR5A 生物学功能 动物模型 

分 类 号：R373.21[医药卫生—病原生物学]