黄芪注射液对失血性休克兔小肠上皮细胞超微结构的影响  

Effect of astragalus membranaceus on the ultrastructure of small intestinal epithelial cells in rabbits with hemorrhagic shock

作　　者：林世清[1] 黑子清[1] 陈秉学[1] 刘卫锋[1] 莫利求[1] 黄文起[1] 

机构地区：[1]中山大学附属第一医院麻醉科,广州510080

出　　处：《中华麻醉学杂志》2003年第6期443-446,共4页Chinese Journal of Anesthesiology

基　　金：广东省中医药管理局资助(102061)

摘　　要：目的 观察黄芪注射液对兔失血性休克小肠超微结构的影响。方法24只大白兔随机分为3组,每组平均8只,分别是H1组:20g/kg黄芪注射液治疗组;H2组:10g/kg黄芪注射液治疗组;C组:生理盐水对照组。复制兔重度失血性休克及复苏的动物模型,实验时取回肠末端组织一份行电子显微镜检查,并测量线粒体二维形态计量学参数和三维形态计量学参数。结果 电镜下可见H1组上皮细胞柱状排列紧密,微绒毛整齐,线粒体丰富,无肿胀;H2组上皮细胞核缩小,微绒毛细、短、肿胀、排列不整,线粒体轻度肿胀,嵴不清,内质网扩张;C组细胞间隙增宽,可见大量的凋亡细胞,微绒毛短、粗、肿胀,部分脱落,线粒体肿胀,嵴断裂,内质网显著扩张,部分呈币状。小肠上皮细胞线粒体体视学参数显示C组上皮细胞单位胞浆内线粒体少,肿胀;H2组的线粒体数目减少,有较明显肿胀;H1组线粒体丰富,肿胀轻微。结论 黄芪注射液对失血性休克损伤兔小肠上皮细胞超微结构有保护作用,并呈一定的量效关系。Objective To investigate the effect of astragalus membranaceus on the ultrastructure of small intestinal epithelial cells in rabbits with hemorrhagic shock. Methods Twenty-four New Zealand white rabbits of both sexes weighing 2-3 kg were randomly divided into 3 equal groups : (A) control group received normal saline iv ( n = 8); (B) group HI received astragalus membranaceus 20 mg· kg-1 iv ( n = 8); ( C) group H2 received astragalus membranaceus 10 mg·kg-1 iv ( n = 8 ). Animal model of hemorrhagic shock-resuscitation was established according to Wigger's. A strip of small intestine, 10 cm in length was taken from distal end of ileum for electron microscopic examination. The two-dimensional structural parameters and three-dimensional structural parameters of mitochondria were calculated. Results (1) Morphological changes of small intestine : in group H1 epithelial cells were orderly arranged, with relatively normal mitochondria and intestinal villi were slender and orderly; in group H2 the nuclei in epithelial cells were dwindled, the intestinal villi were thin and short and unorderly arranged with slightly swelled mitochondria and blurring ridges. Endoplasmatic reticulum was dilated; in group C the gaps between epithelial cells widened. There were a lot of apoptotic cells. Microvilli were thin and short and swelled. Mitochondria were swelled with broken ridges. Endoplasmatic reticulum was severely dilated. (2) Structural parameters of mitochondria : in group C there were least mitochondria and the swelling of mitochondria was severe; in group H1 there were plenty of mitochondria and the swelling was slightest; in group H2 the changes in mitochondria were between group C and H1. Conclusion Astragalus membranaceus can protect small intestine from ischemia/reperfusion injury in a dose-dependent manner.

关 键 词：失血性休克 黄芪注射液 中药制剂 小肠上皮细胞 超微结构 

分 类 号：R285.5[医药卫生—中药学]