酶法转化D,L-苄基海因制备N-氨甲酰-D-苯丙氨酸  

Enzymatic conversion of D,L-benzylhydantoin to N-carbamoyl-D-phenylalanine

作　　者：贾红华[1] 韦萍[1] 周华[1] 欧阳平凯[1] 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京210009

出　　处：《现代化工》2003年第6期24-27,共4页Modern Chemical Industry

基　　金：国家"十五"攻关资助项目 (2 0 0 1BA70 8B0 2 - 0 6)

摘　　要：研究了间歇酶转化和间歇补料酶转化制备N-氨甲酰 -D-苯丙氨酸的过程 ,发现在D ,L-苄基海因 (D ,L-BH)的投入量为 2 0g/L、菌泥与D ,L-BH的质量比为 1∶1的间歇酶转化过程中 ,产物的得率接近 1 0 0 % ,而在初始D ,L-BH的投入量为2 0g/L和菌泥与D ,L-BH之比为 1∶1的间歇补料酶转化过程中 ,则发现在补料的过程中其转化率一直维持在 60 %～ 70 % ,直到底物质量浓度达 50g/L。最终菌泥与D ,L-BH的实际质量比达到了 1∶2 .5 ,提高了酶的利用率。The processes of batch and fed-batch enzymatic conversion to produce N-carbamoyl-D-phenylalanine were studied. It was found that the yield of N-carbamoyl-D-phenylalanine was near 100% in the batch enzymatic conversion process when the concentration of substrate was 20 g/L and the ratio of cell to substrate was 1:1. The yield was 60%-70% in the fed-batch enzymatic r conversion process until the concentration of substrate was from 20 g/L to 50 g/L. Finally, the ratio of cell to substrate was 1:2.5, so the cells was used adequately.

关 键 词：D L-苄基海因 D-苯丙氨酸 N-氨甲酰-D-苯丙氨酸 D-海因酶 

分 类 号：TQ463[化学工程—制药化工]