放射及复合烧伤大鼠腹腔灌洗液对骨髓造血祖细胞生长的影响  

Effects of abdominal lavage fluid from rats with radiation injury and combined radiation-burn injury on growth of hematopoietic progenitor cells

作　　者：冉新泽[1] 粟永萍[1] 程天民[1] 郭朝华[1] 刘晓宏[1] 屈纪富[1] 艾国平[1] 林远[1] 

机构地区：[1]第三军医大学预防医学系全军复合伤研究所,重庆400038

出　　处：《中华放射医学与防护杂志》2003年第3期145-147,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金重点资助项目(30 2 30 36 0396 70 2 89)

摘　　要：目的　观察放射损伤、烧伤及放烧复合伤腹腔灌洗液对骨髓体外造血的影响。方法60 Coγ射线全身照射 1 2Gy、5kW溴钨灯致大鼠背部 30 %TBSAⅢ度烧伤。伤后 3 ,1 2 ,2 4 ,48和 72h无菌抽取腹腔灌洗液 ,按 40 μg ml加入到骨髓红系、粒系培养体系中培养。 结果　伤后 3 ,1 2 ,2 4 ,48和72h,烧伤组和复合伤组的CFU E、BFU E和CFU GM培养集落数均显著高于正常对照组 (P <0 0 1 ) ,且以伤后 2 4h最高 ,烧伤组高达 2 1 5 7%、2 0 2 3 %和 2 4 1 2 % ,复合伤组为 1 88 1 %、1 4 7 7%和2 0 4 6 %。但当加入放射损伤大鼠腹腔灌洗液时 ,仅有很少的CFU E、BFU E和CFU GM集落生长。结论　烧伤与放烧复合伤后的腹腔灌洗液对骨髓红系、粒系造血均有明显的促进作用 。Objective\ To observe the effects of abdominal lavage fluid from rats with radiation injury,burn injury and combined radiation\|burn injury on growth of hematopoietic progenitor cells. Methods\ Rats were irradiated with 12 Gy whole body 60 Co γ rays and/or inflicted with 30% TBSA III degree burn with a 5 kW bromotungsten lamp.The abdominal lavage fluid from the animals was collected at 3 h,12 h,24 h,48 h,72 h after injury, separately. Then each of the lavage fluid samples was added to the medium of cultured erythrocyte progenitor cells (CFU\|E,BFU\|E) or of cultured granulocyte\|macrophage progenitor cells (CFU\|GM) at a final concentration of 40 μg/ml. Results\ The colony numbers of CFU\|E,BFU\|E and CFU\|GM formed after addition of the lavage fluid from rats with burn injury or combined radiation\|burn injury were significantly higher than those from normal rats at 3 h,12 h,24 h,48 h and 72 h after injury and reached their peak values at 24 h after injury (215 7%,202 3% and 241 2%,respectively from solely burned rats;188 1%,147 7% and 204 6%,respectively from rats with combined radiation\|burn injury as compared with that of normal rats).However,only few CFU\|E,BFU\|E or CFU\|GM colonies were found after addition of the lavage fluid from solely irradiated rats. Conclusions\ The abdominal lavage fluid from rats with sole burn injury or combined radiation\|burn injury promotes growth of erythrocyte and granulocyte progenitor cells.On the contrary,the lavage fluid from solely irradiated rats shows inhibiting effects.\;

关 键 词：放射损伤 复合烧伤 腹腔灌洗液 骨髓造血祖细胞 造血功能障碍 大鼠 

分 类 号：R818[医药卫生—放射医学] R644[医药卫生—临床医学]