小鼠骨髓程序移植时每次移植回巢的确证  

Evidence of homing of each fraction of bone marrow cells after scheduled transplatation in mice

作　　者：孙苏平[1] 蔡建明[1] 项莺松[1] 黄定德[1] 赵芳[1] 高建国[1] 杨如俊[1] 

机构地区：[1]第二军医大学放射医学教研室,上海200433

出　　处：《中华放射医学与防护杂志》2003年第3期157-159,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目(39770 32 9396 0 0 0 77)

摘　　要：目的　进一步证实骨髓程序移植时每次移植后都有移植细胞的回巢。方法　采用同种同基因 (H 2Kd) +同种异基因 (H 2Kb)小鼠骨髓细胞在不同时间点的混合程序移植模型 ,以及流式细胞仪检测每一次移植的异基因骨髓细胞的回巢情况。结果　程序移植时 ,每次异基因骨髓细胞移植后在骨髓内均有较高的H 2Kb 阳性细胞回巢 ,并以第 2次移植为最高 (P <0 0 1 )。H 2Kb 细胞在脾中的回巢随着移植次数的增加而减少 ,与在骨髓中的回巢规律不同。结论　程序移植可能是通过多次移植后的每一次回巢 ,充分利用了不断腾出的龛位 。Objective\ To identify homing of bone marrow cells after every fractionation during scheduled transplantation. Methods\ The recipient mice were transplanted with homologous (H 2K d) and allogenic(H 2K b) mouse bone marrow cells after lethal irradiation,and the homing status of allogenic bone marrow cells in host bone marrow and spleen was observed. Results\ A quantity of allogenic homed cells were observed in host bone marrow,and the percentage of homing cells in second fraction was the highest in all groups ( P <0 01).The allogenic homed cells in spleen declined along with increase of the number of fraction,suggesting that regulation of homing to spleen was different from that to bone marrow. Conclusion\ In scheduled bone marrow transplantation niche may be more effectively utilized and thus transplation efficiency be enhanced.\;

关 键 词：骨髓程序移植 回巢 龛位 动物实验 

分 类 号：R457.7[医药卫生—治疗学]