抗纤复方含药血清对HSC-LI90细胞基质金属蛋白酶及其抑制因子基因表达的影响  

Effect of Anti-fibrosis Compound Contained Serum on Matix Metalloproteinase and Tissue Inhibitor of Metalloprotenase-1 Gene Expression of HSC-LI90 Cells

作　　者：赵钢[1] 王灵台[1] 陈建杰[1] 张斌[1] 

机构地区：[1]上海中医药大学附属曙光医院肝科,上海200021

出　　处：《中国实验方剂学杂志》2003年第4期31-33,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金 ( 3 9670 90 6)

摘　　要：目的 :观察抗纤复方含药血清对肝星形细胞 (HSC)LI90细胞 (HSC LI90 )基质金属蛋白酶及其抑制因子基因表达的影响。方法 :以 0 5、2 0、4 0g kg等不同剂量抗纤复方灌胃大鼠制备的含药血清 ,作用HSC LI90细胞 4 8h ,应用Northern印迹杂交的方法 ,检测膜型基质金属蛋白酶、基质金属蛋白酶 2及其抑制因子 1(TIMP 1)基因的表达 ,并用酶图法检测基质金属蛋白酶 2的活性。结果 :抗纤复方含药血清抑制HSC LI90细胞TIMP 1,增加HSC LI90细胞膜型基质金属蛋白酶基因表达 ,对基质金属蛋白酶 2基因表达及其活性没有影响。结论 :抗纤复方抑制HSC LI90细胞TIMP 1基因表达水平 ,促进胶原降解 ,可能是抗肝纤维化的作用机制之一。Objective:To observe the in vitro effect of anti\|fibrosis Compound contained (AFC) serum on matix metalloproteinase and tissue inhibitor of metalloprotenase\|1 (TIMP 1) mRNA levels of HSC LI90 cell. Methods:HSC LI90 cells were exposed in different doses of AFC contained serum(0.5,2.0 4.0g/kg) for 48 hours.The gene expressions of matix metalloproteinase\|2(MMP\|2) and membrane type matrix metalloproteinase (MT\|MMP) and TIMP 1 were investigated by Northern blot,The gelatinase activity of MMP\|2 was examined by zymography.Results:AFC contained serum could inhibit the TIMP 1 and increased the MT\|MMP the gene expressions of HSC LI90,The expressions of MMP\|2 and MMP\|2 activity were not affected by different doses AFC contained serum.Conclusions:AFC contained serum can decrease TIMP 1 mRNA expression of HSC LI90 cells.

关 键 词：抗纤复方 HSC-LI90 基质金属蛋白酶 TIMP-l Northern印迹杂交 酶图法 

分 类 号：R285.5[医药卫生—中药学]