血液中一种新的“濒亡标志物”(醇脱氢酶同工酶)的鉴定  

A new"death marker"presented in serum:alcohol dehydrogenase isoenzyme

作　　者：宓庆梅[1] 于嘉屏[1] 王爱华[1] 

机构地区：[1]第二军医大学附属长征医院实验诊断科,上海200003

出　　处：《临床检验杂志》2003年第4期203-205,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：目的　鉴定血清乳酸脱氢酶 (LD)同工酶检测中出现的第 6条带“LD6”的蛋白质性质。方法　用琼脂糖凝胶电泳分离、提纯“LD6” ,聚丙烯酰胺凝胶电泳检测“LD6”相对分子质量 ,免疫转移印迹法鉴定人血清中“LD6”抗原性。结果　测定人血清LD同工酶时出现的第 6条带“LD6”为醇脱氢酶 (alcoholdehydrogenase ,AD)同工酶 ,相对分子质量为 80 0 0 0 ,用western免疫固定转印法鉴定核实。正常人肝组织匀浆、肝癌组织匀浆作琼脂糖凝胶电泳分离、提纯“LD6” ,聚丙烯酰胺凝胶电泳检测 ,表明肝组织匀浆、肝癌组织匀浆的“LD6”与血清“LD6”为同一物质。 1 0 0例患者有 37例血清含有AD ,其中 34例AD活性≥LD总活性7.9% ,病人 1周内死亡 ,死亡率达 91 .9%。结论　正常人血清内不易检测出AD活性 ,而正常肝组织内有强的AD Ⅱ活性。当肝脏受到严重损害时 ,AD从肝细胞内逸出 ,释放入血 ,导致血清AD活性明显升高。血清中AD活性上升可能是一种新的“濒亡标志物”。Objective To characterize the component of lactate dehydrogenase'isoenzyme 6'presented in serum.Methods Serum LD6 was isolated and purified by agarose gel electrophoresis.The molecular mass of LD6 was identified by SDS PAGE and its antigenicity was confirmed by western blot.Results LD6 is alcohol dehydrogenase isoenzyme Ⅱ(AD-Ⅱ) with 80 000 of Mr.The characteristics of the'LD6'presented in homogenate of normal liver and liver cancer tissue were similar to those of'LD6'presented in serum.Conclusions The activity of'LD6'in normal human serum is too low to be measured.However,very high LD and AD activity in normal liver tissue is revealed and can be detected easily by routine agarose gel electrophoresis.The high AD activity is displayed without lactate as substrate and could be enhanced by ethanol added to the substrates.The activity of LD isoenzymes in 100 serum was from 1 340.1～3 016.5 U/L,and AD band was found in 37 cases. Thirty-four ases whose AD activity was higher than total LD activity died in one week.Therefore,we consider the rised AD activity in serum was a death marker.

关 键 词：濒亡标志物 血液 醇脱氢酶同工酶 乳酸脱氢酶同工酶 电泳 免疫印迹 

分 类 号：R446.1[医药卫生—诊断学] R339.39[医药卫生—临床医学]