甲基莲心碱对长春新碱诱导人胃癌细胞凋亡的影响  

Effects of neferine on gastric carcinoma apoptosis induced by vincristine

作　　者：石书红[1] 庄英帜[1] 曹建国[2] 

机构地区：[1]南华大学第一附属医院肿瘤内科,衡阳421001 [2]南华大学第一附属医院肿瘤研究所,衡阳421001

出　　处：《中国药理学通报》2003年第8期928-930,共3页Chinese Pharmacological Bulletin

摘　　要：目的　观察甲基莲心碱 (Nef)在体外对长春新碱诱导人胃癌细胞凋亡的影响。方法　采用MTT比色法检测药物的细胞毒性作用 ,并用流式细胞术、AO/EB双重荧光染色法和TUNEL法测定甲基莲心碱对长春新碱诱导人胃癌细胞凋亡率的影响。结果　 2 5、5、1 0 μmol·L- 1 Nef能增强长春新碱对人胃癌细胞 (SGC790 1 )增殖的抑制作用 ;1 0 μmol·L- 1 Nef能增强长春新碱 (0 1、0 5、2、4mg·L- 1 )诱导SGC790 1细胞凋亡。结论　甲基莲心碱能增强长春新碱诱导人胃癌细胞凋亡 ,推测其为一种低毒高效的化疗增敏剂。AIM To study the effects of neferine on gastric car cinoma apoptosis induced by vincristine in vitro. METHODS Cytotoxicity assay was tested by MTT method. The influence of neferine to affect vincristine to induce gastric carcinoma apoptosis was detected by PI staining flow cytometry, AO/EB double fluorescence stain and terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL). RESULTS 2 5, 5,10 μmol·L -1 neferine enhanced vincristine to inhibit the proliferation of SGC7901 cells; 10 μmol·L -1 neferine enhanced vincristine (0 1, 0 5, 2, 4 mg·L -1 ) to induce SGC7901 cells apoptosis. CONCLUSIONS Neferine enhanced vincristine to induce gastric carcinoma cells apoptosis. It is inferred a kind of low poisonous and high effective chemosenstizers.

关 键 词：甲基莲心碱 长春新碱 化疗增敏剂 胃癌细胞 凋亡 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]