青岛文昌鱼DAD1样蛋白基因的克隆和同源性分析  

Cloning and homology analysis of dad 1-like gene in Qingdao amphioxus

作　　者：杨永杰[1] 张燕君[1] 王振光[1] 张培军[2] 石德利[1] 张红卫[1] 

机构地区：[1]山东大学生命科学学院发育研究所,山东济南250100 [2]中国科学院青岛海洋研究所,山东青岛266071

出　　处：《山东大学学报（理学版）》2003年第3期108-111,共4页Journal of Shandong University(Natural Science)

基　　金：国家自然科学基金 (3 0 0 70 0 94) (3 0 2 70 693 ) ;山东省自然科学基金 (L2 0 0 0D0 3 )

摘　　要：dad1基因为一种内源性细胞凋亡抑制子基因 ,在发育中可能执行着重要功能 .我们通过对青岛文昌鱼 18小时神经胚cDNA文库进行测序 ,获得文昌鱼dad1样基因的 2个cDNA片断 ,经拼接首次得到含有完整读框的文昌鱼dad1样基因的cDNA序列 ,其演绎的氨基酸序列与人、鼠、鸡、非洲爪蟾、果蝇、线虫、扁豆、番茄的DAD1蛋白的同源性分别为 73.5 %、73.5 %、6 7.5 %、70 .9%、6 7.5 %、5 5 .5 %、4 1.9%、4 1.8% .结果证实青岛文昌鱼dad1样基因在进化上具有高度的保守性 。By sequencing a 18h-neurulae cDNA library of Qingdao amphioxus, we obtained two expressed sequence tags(ESTs) of dad1-like gene,and merged them together into a completed cDNA sequence as well.The deduced amino acid sequence of dad1-like gene was analyzed and compared with the homology proteins of it in vertebrate,invertebrate and plant.The identities compared with that of Human, Mouse, Chick, African clowed frog, Fruit fly, C.elegans, Pea,Tomato's is 73.5%, 73.5%,67.5%, 70. 9%, 67.5%, 55.5%,41.9%,41.8% respectively. The results showed that dad1 gene is very conserved in evolution, implying that dad1 gene is very important in development.

关 键 词：文昌鱼 dud1基因 进化 

分 类 号：Q754[生物学—分子生物学]