水稻蔗糖转化酶基因的克隆及其功能的初步探讨  

Cloning and Characterization of a cDNA Encoding Putative Neutral/Alkaline Invertase from Rice (Oryza sativa L.)

作　　者：姜立智[1] 林长发[1] 梁宗锁[2] 卫春[1] 杨金水[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所,上海200433 [2]西北农林科技大学生命科学学院,杨凌712100

出　　处：《复旦学报（自然科学版）》2003年第4期588-592,共5页Journal of Fudan University：Natural Science

基　　金：国家重点基础研究发展规划资助项目(2001CB108805)

摘　　要：根据抑制消减杂交(suppressionsubtractivehybridization,SSH)方法分离到在水稻叶片中高表达的蔗糖转化酶基因片段,根据水稻基因组顺序设计引物分离到全长cDNA.测序结果表明该cDNA长度为1937bp,推测编码一个627个氨基酸的中性/碱性蔗糖转化酶.Alignment分析显示该推测蛋白质与已知的多个蔗糖转化酶具有很高的同源性.半定量PCR检测证实它在叶中的表达强于在根、花药和幼穗等组织中的表达,胁迫处理(盐和低温)使其表达上调.A fragment which is higher expression in the leaves of rice was isolated using suppression subtractive hybridization (SSH). It is shown to be homologous with the invertase gene. Primers were then designed to obtain its complete cDNA. Sequencing indicatesd that Osinv(Oryza sativa invertase)contains 1 937 bp which encoding a putative protein with 627 amino acids. Sequence alignment showed that the deduced proteins have high homology to neutral/alkalin invertases in other plants. RTPCR was performed to reveal that Osinv is abundant in leaf, rather than in root, anther and young panicle. Furthermore, the expression of Osinv is accelerated after stress treat ( such as salt and cold).

关 键 词：抑制消减杂交 蔗糖转化酶 基因克隆 RT-PCR 水稻 

分 类 号：Q943.2[生物学—植物学]