抗原致敏大鼠离体肺片VCAM-1表达及药物调节作用  

Expression of vascular cell adhesion molecule-1 in lung slices from antigen-sensitized rats and pharmacological modulation

作　　者：杨晓红[1] 魏尔清[1] 

机构地区：[1]浙江大学医学院药理学教研室,浙江杭州310031

出　　处：《浙江大学学报（医学版）》2003年第4期319-322,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金资助项目 (39770 86 5 )

摘　　要：目的 :验证致敏大鼠离体肺片血管细胞黏附分子 - 1(VCAM- 1)表达特点及药物调节作用。方法 :体外培养卵白蛋白 (OVA)致敏大鼠肺片 ,在药物处理后以 OVA攻击 3h,测定肺片 VCAM- 1的相对表达量。结果 :致敏大鼠离体肺片的 VCAM- 1表达增加 ,但 OVA攻击不能使其进一步增高。地塞米松和 ONO- 10 78能下调致敏诱导的VCAM- 1表达增高 ,而 SR- 14 0 333无此作用。结论 :动物致敏后离体肺片 VCAM- 1表达增高 ,抗原攻击不能上调其表达 ;抗炎症药对 VCAM- 1表达的作用不同 ,地塞米松和 ONO- 10 78抑制其表达 ,而 SR- 14 0 333无作用。Objective: To investigate the expression of vascular cell adhesion molecule-1(VCAM-1) in lung slices from antigen-sensitized rats and the modulation by drugs. Methods: In isolated lung slices from ovalbumin(OVA)-sensitized rats,the relative expression of VCAM-1 was determined after drug treatment and OVA challenge. Results: The expression of VCAM-1 was enhanced in the sensitized rat lungs,and OVA challenge did not further increase the expression. Glycocorticosteroid dexamethasone and leukotriene cysLT receptor antagonist ONO-1078 inhibited the expression,but tachykinin NK-1 receptor antagonist SR-140333 had no such effect. Conclusion: VCAM-1 expression is enhanced in the sensitized rat lungs,and antigen challenge does not further up- regulate the expression. Anti-inflammatory drugs have different effects on VCAM-1 expression. Dexamethasone and ONO-1078,but not SR-140333,can inhibit the expression.

关 键 词：细胞黏着分子 白三烯类/副作用 受体速激肽/拮抗剂和抑制剂 地塞米松/药理学 哮喘/病因学 大鼠 

分 类 号：R562.25[医药卫生—呼吸系统]