利用非侵入性光学成像技术监测在体肿瘤的生长  

In vivo Optical Imaging of the Tumor Growth

作　　者：陆锦玲[1] 李鹏程[1] 朱 骆清铭[1] 

机构地区：[1]华中科技大学生物医学光子学教育部重点实验室,中国湖北武汉430074

出　　处：《激光生物学报》2003年第4期282-287,共6页Acta Laser Biology Sinica

基　　金：国家自然科学基金重点项目(59836240);中国博士后科学基金(2002031256)

摘　　要：利用电穿孔技术将gfp表达质粒转染于SP2/0细胞,通过G418筛选获得稳定表达GFP的小鼠骨髓瘤细胞株SP2/0-GFP细胞。将稳定的转化细胞移植于同系BALB/c小鼠后腿、耳部皮下及腹腔成瘤,每隔一天对小鼠肿瘤成像,跟踪肿瘤的生长过程。结果表明,探测到的GFP荧光强度和发光范围与肿瘤的生长或消亡相关,从而证明可以利用GFP作为肿瘤细胞的标记,结合光学成像技术,对肿瘤生长过程实时追踪。The myeloma cell line, SP2/0 from the BALB/c mouse, was engineered to stably express highlevels of the green fluorescent protein (GFP) by electroporation technology and selected with G418. The GFPexpressing tumor cells(SP2/0-GFP) were implanted into BALB/c mouse, and optical images visualized realtime tumor growth. The results show that the fluorescence of GFP is comparably with the tumor growth and/or depress. The GFP imaging technology presented in this report will facilitate studies of tumor growth.

关 键 词：绿色荧光蛋白(GFP) 电穿孔 肿瘤 在体光学成像 

分 类 号：Q63[生物学—生物物理学]